Fig. 1

Comparison between growth of E. coli BW25113 TraDIS-Xpress library and P. aeruginosa PA14, either alone or in co-cultures and in the presence or absence of 4% glucose. A Cultures (n = 3) were grown in LB for 24 h, as described in Materials and Methods then diluted and spread on LBA plates supplemented with appropriate antibiotics. Colonies were counted after overnight incubation at 37 °C. Bars indicate mean values and points indicate individual data. The p-value result of a two-way T-test comparing average CFU/mL of E. coli and P. aeruginosa under each condition is given above each pair of bars. B The colour of a culture after 24 h incubation provide an approximate indication of relative P. aeruginosa cell numbers. Cultures dominated by E. coli remained yellow-beige, while those with higher numbers of P. aeruginosa became blue-green due to production of pyocyanin