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Optimisation of rhamnolipids produced byPseudomonas aeruginosa 181 using Response Surface Modeling

Annals of Microbiology volume 57, pages 571–575 (2007)Cite this article

Abstract

This work investigated the optimisation of the fermented culture medium for maximisation of rhamnolipids production produced byPseudomonas aeruginosa 181 using Response Surface Modeling (RSM). A two full factorial central composite experimental design was used in the design of experiments and in the analysis of results. This procedure limited the number of actual experiments performed while allowing for possible interactions between the parameters (pH, stirring rate, casamino acid concentration and incubation period) on the production of biosurfactants. Production carried out at larger volumes of one litre using Bioreactor under RSM-optimised conditions yielded 3.61 g l−1 of products after purification by acid precipitation.

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Authors and Affiliations

  1. Department of Basic Medical Sciences, Faculty of Nursing, International Islamic University Malaysia, P.O Box 141, 25710, Kuantan, Pahang Darul Makmur, Malaysia

    Laith Issa Yassin Al-Araji

  2. Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM, Selangor, Malaysia

    Raja Noor Zaliha Raja Abd Rahman & Abu Bakar Salleh

  3. Faculty of Science, University Putra Malaysia, 43400 UPM, Selangor, Malaysia

    Mahiran Basri

Authors
  1. Laith Issa Yassin Al-Araji
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  2. Raja Noor Zaliha Raja Abd Rahman
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  3. Mahiran Basri
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  4. Abu Bakar Salleh
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Correspondence to Laith Issa Yassin Al-Araji.

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Al-Araji, L.I.Y., Rahman, R.N.Z.R.A., Basri, M. et al. Optimisation of rhamnolipids produced byPseudomonas aeruginosa 181 using Response Surface Modeling. Ann. Microbiol. 57, 571–575 (2007). https://doi.org/10.1007/BF03175357

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  • DOI: https://doi.org/10.1007/BF03175357

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Annals of Microbiology

ISSN: 1869-2044

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