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A spreading colony formed method for rapid evaluation of dicarboximide fungicides resistance level of field tobacco brown spot disease

Annals of Microbiology volume 59, pages 173–177 (2009)Cite this article

Abstract

A new method (spreading colony formed method) for rapidly identifying and evaluating the dicarboximide fungicides resistance level of field tobacco spot brown disease caused byAlternaria longipes was developed. Two typical colonies with distinct differences in colony morphology on media containing 5 μg/ml dicarboximide fungicides dimethachlon (CAS registration number: 24096-53-5) were discovered by using this method. The two typical colonies were named spreading colony and dense pad colony, respectively. Isolates (250) ofA. longipes were quickly separated by this method, and their growth properties (including the sensitivity to dimethachlon, the cross-resistance to phenylpyrroles fludioxonil and hyphal development) were examined. Our results indicated that (1) monospore isolates from spreading colonies and dense pad colonies were respectively resistant and sensitive to dimethachlon; (2) resistant and sensitive isolates formed respectively spreading colonies and dense pad colonies on dimethachlon media. Furthermore, molecular experiments confirmed the spreading colony formed method reliable. In conclusion, field resistant isolates and resistant situation in population level of field tobacco spot brown disease could be exactly and timely determined and evaluated by spreading colony formed method.

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Authors and Affiliations

  1. Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, 650091, Kunming

    Yiyong Luo, Jinkui Yang, Zebao Lu, Wei Bi & Keqin Zhang

  2. Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, 650091, Kunming

    Yiyong Luo, Jinkui Yang, Zebao Lu, Wei Bi & Keqin Zhang

  3. Yunnan Academy of Tobacco Science, 653100, Yuxi, China

    Mingliang Zhu

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  1. Yiyong Luo
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  2. Mingliang Zhu
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  3. Jinkui Yang
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  4. Zebao Lu
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  5. Wei Bi
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  6. Keqin Zhang
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Correspondence to Keqin Zhang.

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Luo, Y., Zhu, M., Yang, J. et al. A spreading colony formed method for rapid evaluation of dicarboximide fungicides resistance level of field tobacco brown spot disease. Ann. Microbiol. 59, 173–177 (2009). https://doi.org/10.1007/BF03175617

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Annals of Microbiology

ISSN: 1869-2044

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