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Table 1 Primers used for amplification and sequencing of the type III effector proteins-encoding genes

From: Distribution of the type III effector proteins-encoding genes among nosocomial Pseudomonas aeruginosa isolates from Bulgaria

Primers

Target gene

Sequence (5′–3′)

Product size (bp)

Position on P. aeruginosa PAO1 chromosome (locus)

Annealing temperature (ºC)

Source

Pairs for PCR

 exoS-F

exoS

CTT GAA GGG ACT CGA CAA GG

504

PA3841

60

Lanotte et al. 2004

 exoS-R

 

TTC AGG TCC GCG TAG TGA AT

    

 exoT-F

exoT

CAA TCA TCT CAG CAG AAC CC

1159

PA0044

58

Finnan et al. 2004

 exoT-R

 

TGT CGT AGA GGA TCT CCT G

    

 exoY-F

exoY

TAT CGA CGG TCA TCG TCA GGT

1035

PA2191

64

Finnan et al. 2004

 exoY-R

 

TTG ATG CAC TCG ACC AGC AAG

    

 exoU-F

exoU

GGG AAT ACT TTC CGG GAA GTT

428

PS14 within P. aeruginosa PA14 pathogenicity island PAPI-2

60

Allewelt et al. 2000

 exoU-R

 

CGA TCT CGC TGC TAA TGT GTT

    

Sequencing primers

 exoS-F-seq

exoS

ATG CAT ATT CAA TCG CTT CA

   

Strateva 2008

 exoS-R-seq

exoS

CGA CCG GTC AGG CCA GAT

   

Strateva 2008

 exoU-R-seq1

exoU

TCA TGT GAA CTC CTT ATT

   

Strateva 2008

 exoU-R-seq2

exoU

CGA GAG AAG CGA AGG TAT GA

   

Strateva 2008

  1. PCR Polymerase chain reaction, F forward, R reverse
  2. exoS exoenzyme S-encoding gene, exoT exoenzyme T-encoding gene, exoY exoenzyme Y-encoding gene, exoU exoenzyme U-encoding gene