Primers | Target gene | Sequence (5′–3′) | Product size (bp) | Position on P. aeruginosa PAO1 chromosome (locus) | Annealing temperature (ºC) | Source |
---|---|---|---|---|---|---|
Pairs for PCR | ||||||
exoS-F | exoS | CTT GAA GGG ACT CGA CAA GG | 504 | PA3841 | 60 | Lanotte et al. 2004 |
exoS-R | TTC AGG TCC GCG TAG TGA AT | |||||
exoT-F | exoT | CAA TCA TCT CAG CAG AAC CC | 1159 | PA0044 | 58 | Finnan et al. 2004 |
exoT-R | TGT CGT AGA GGA TCT CCT G | |||||
exoY-F | exoY | TAT CGA CGG TCA TCG TCA GGT | 1035 | PA2191 | 64 | Finnan et al. 2004 |
exoY-R | TTG ATG CAC TCG ACC AGC AAG | |||||
exoU-F | exoU | GGG AAT ACT TTC CGG GAA GTT | 428 | PS14 within P. aeruginosa PA14 pathogenicity island PAPI-2 | 60 | Allewelt et al. 2000 |
exoU-R | CGA TCT CGC TGC TAA TGT GTT | |||||
Sequencing primers | ||||||
exoS-F-seq | exoS | ATG CAT ATT CAA TCG CTT CA | Strateva 2008 | |||
exoS-R-seq | exoS | CGA CCG GTC AGG CCA GAT | Strateva 2008 | |||
exoU-R-seq1 | exoU | TCA TGT GAA CTC CTT ATT | Strateva 2008 | |||
exoU-R-seq2 | exoU | CGA GAG AAG CGA AGG TAT GA | Strateva 2008 |