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Table 4 Central composite rotational design (CCRD) for optimization of three variables in experimental values for enzymatic activity by isolated Bacillus lehensis CGII

From: Bacillus lehensis—an alkali-tolerant bacterium isolated from cassava starch wastewater: optimization of parameters for cyclodextrin glycosyltransferase production

Run

Y a1

Y b2

Y c3

Enzymatic activity (g L−1)

1

5

5

7.5

53.12

2

5

5

12.5

36.89

3

5

10

7.5

57.55

4

5

10

12.5

44.27

5

10

5

7.5

38.37

6

10

5

12.5

20.66

7

10

10

7.5

56.07

8

10

10

12.5

36.89

9

3.3

7.5

10

69.65

10

11.7

7.5

10

48.25

11

7.5

3.3

10

32.31

12

7.5

11.7

10

67.88

13

7.5

7.5

5.8

63.45

14

7.5

7.5

14.2

37.62

15

7.5

7.5

10

95.32

16

7.5

7.5

10

97.34

17

7.5

7.5

10

97.38

  1. aCassava starch concentration
  2. bNitrogen source concentration
  3. cSodium carbonate concentration