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Table 3 Analysis of variance, showing the effect of the variables interactions on the response Y1 and Y2 of the central composite design

From: Optimal culture conditions for producing conjugated linoleic acid in skim-milk by co-culture of different Lactobacillus strains

Source

Sum of squares

Degree of freedom

Mean square

F value

Prob > F

La–Lp (Y1); R 2 = 0.9119. Adeq precision = 10.339. CV(%) = 24.69

Model

2.216E + 005

14

15,828.22

10.35

<0.0001***

A

13,380.04

1

13,380.04

8.75

0.0104*

B

119.13

1

119.13

0.078

0.7843

C

1,034.53

1

1,034.53

0.68

0.4246

D

8,034.71

1

8,034.71

5.25

0.0397*

AB

7,165.18

1

7,165.18

4.98

0.0425*

AC

1,769.88

1

1,769.88

1.16

0.3002

AD

55.13

1

55.13

0.036

0.8521

BC

747.20

1

747.20

0.49

0.4960

BD

4.95

1

4.95

3.237E-003

0.9554

CD

2,728.50

1

2,728.50

1.78

0.2030

A2

38,707.43

1

38,707.43

25.31

0.0002**

B2

55,602.25

1

55,602.25

36.36

<0.0001***

C2

20,870.94

1

20,870.94

13.65

0.0024**

D2

1.471E + 005

1

1.471E + 005

96.15

<0.0001***

Lack of fit

21,267.14

10

2,126.71

58.91

0.0007

Pure error

144.41

4

36.10

  

Cor total

2.430 E + 005

28

   

La–St (Y2); R 2 = 0.8556. Adeq precision = 8.691. CV(%) = 21.80

Model

55,338.32

14

3,952.74

5.92

0.0010**

A

7,049.48

1

7,049.48

10.56

0.0058**

B

4,049.48

1

4,049.48

6.07

0.0273*

C

4,915.49

1

4,915.49

7.37

0.0168*

D

338.99

1

338.99

0.51

0.4877

AB

21.34

1

21.34

0.032

0.8606

AC

2,596.92

1

2,596.92

3.89

0.0686

AD

2,228.31

1

2,228.31

3.34

0.0890

BC

1,574.50

1

1,574.50

2.36

0.1468

BD

72.93

1

72.93

0.11

0.7459

CD

1,820.30

1

1,820.30

2.73

0.1209

A2

9,842.64

1

9,842.64

14.75

0.0018**

B2

25,753.17

1

25,753.17

38.59

<0.0001***

C2

1,747.57

1

1,747.57

2.62

0.1279

D2

1,636.28

1

1,636.28

2.45

0.1397

Lack of fit

9,028.51

10

902.85

11.50

0.0155

Pure error

314.17

4

78.54

  

Cor total

64,681.00

28

   
  1. *P < 0.05, **P < 0.01, ***P < 0.0001