From: Evaluation of native potential probiotic bacteria using an in vitro ruminal fermentation system
Variables | Substrate | Control fermentation | Ruminal bacterial strains | |||||
---|---|---|---|---|---|---|---|---|
21C | 22C | 62C | 60C | 50C | 55C | |||
VFA concentration (mM) | ||||||||
Acetic acid | Wheat straw | 41.2 (3.1) | 43.9 (2.8) | 41.8 (3.2) | 35.9 (5.4) | 32.6 (0.6) | 44.3 (2.2) | 30.6 (1.6)* |
Oat xylan | 47.0 (5.5) | 49.6 (1.7) | 44.3 (3.5) | 42.7 (1.1) | 48.1 (2.2) | 32.4 (2.7) | 22.1 (21.0)* | |
Cellulose | 38.3 (4.5) | 39.5 (3.2) | 34.5 (3.1) | 31.7 (4.9) | 31.5 (3.5) | 39.8 (0.6) | 27.4 (3.2)* | |
Propionic acid | Wheat straw | 13.9 (1.0) | 14.7 (0.9) | 12.0 (0.5) | 12.1 (2.0) | 9.6 (0.2)* | 15.1 (0.8) | 9.2 (0.4)* |
Oat xylan | 11.8 (0.9) | 11.5 (0.8) | 10.6 (0.7) | 10.2 (0.2) | 11.2 (0.8) | 6.2 (0.3)* | 4.9 (4.9)* | |
Cellulose | 8.8 (1.0) | 9.5 (0.8) | 12.3 (0.4)* | 7.7 (1.3) | 6.4 (0.9)* | 9.3 (0.2) | 5.5 (0.8)* | |
Butyric acid | Wheat straw | 0.3 (0.1) | 16.9 (0.8)* | 13.4 (1.0)* | 11.6 (1.57)* | 11.9 (0.2)* | 16.2 (0.4)* | 7.8 (4.7)* |
Oat xylan | 0.7 (0.2) | 31.0 (0.9)* | 22.6 (2.0)* | 22.3 (0.6)* | 30.4 (2.1)* | 11.5 (9.5) | 13.8 (13.1* | |
Cellulose | 0.1 (0.1) | 24.8 (2.0)* | 17.3 (2.1)* | 16.2 (2.1)* | 18.8 (2.3)* | 25.0 (0.4)* | 16.6 (2.1)* | |
Total VFA | Wheat straw | 55.3 (4.0) | 75.5 (4.5)* | 67.2 (3.7) | 59.6 (9.0) | 54.1 (0.7) | 75.6 (3.3)* | 47.8 (5.0) |
Oat xylan | 59.5 (6.0) | 92.2 (2.2) | 77.5 (6.2) | 75.2 (0.9) | 89.8 (4.8) | 50.17 (6.4) | 40.8 (39.1) | |
Cellulose | 47.2 (5.4) | 73.8 (6.1)* | 64.1 (4.7)* | 55.7 (8.3) | 56.7 (6.8) | 74.1 (0.7)* | 49.5 (6.1) | |
Acetic:propionic ratio | Wheat straw | 2.96 (0.02) | 2.98 (0.01) | 3.49 (0.42)* | 2.99 (0.06) | 3.40 (0.02) | 2.94 (0.02) | 3.34 (0.07) |
Oat xylan | 3.97 (0.39) | 4.31 (0.14) | 4.16 (0.10) | 4.19 (0.08) | 4.30 (0.15) | 5.21 (0.16)* | 4.68 (0.28)* | |
Cellulose | 4.37 (0.12) | 4.18 (0.05) | 2.80 (0.30)* | 4.13 (0.10) | 4.93 (0.19)* | 4.28 (0.01) | 5.00 (0.16)* | |
Final pH | Wheat straw | 5.8 | 5.82 | 5.88* | 5.92* | 5.87 | 5.49* | 5.87 |
Oat xylan | 5.8 | 5.18* | 5.14* | 5.16* | 5.14* | 5.49* | 5.14* | |
Cellulose | 5.8 | 5.50* | 5.51* | 5.52* | 5.51* | 5.49* | 5.50* |