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Fig. 1a–e | Annals of Microbiology

Fig. 1a–e

From: Protoplasting, regeneration and transformation of medicinal mushroom Ganoderma multipileum using succinate dehydrogenase mutation gene as a selection marker

Fig. 1a–e

Protoplasts of Ganoderma multipileum and regeneration on regeneration medium broth (RMB). a Light microscopy of generated protoplasts. b, c Regeneration of protoplasts forms short (b) or long (c) hyphae after incubation for 16 h under constant light illumination. d, e Except for small size or anucleated protoplasts, regenerated protoplasts grow and form longer and wider hyphae, after incubation for 27 h under constant illumination. Bars  5 μm

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