Fig. 1a–eFrom: Protoplasting, regeneration and transformation of medicinal mushroom Ganoderma multipileum using succinate dehydrogenase mutation gene as a selection markerProtoplasts of Ganoderma multipileum and regeneration on regeneration medium broth (RMB). a Light microscopy of generated protoplasts. b, c Regeneration of protoplasts forms short (b) or long (c) hyphae after incubation for 16 h under constant light illumination. d, e Except for small size or anucleated protoplasts, regenerated protoplasts grow and form longer and wider hyphae, after incubation for 27 h under constant illumination. Bars 5 μmBack to article page