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Fig. 2a,b | Annals of Microbiology

Fig. 2a,b

From: Protoplasting, regeneration and transformation of medicinal mushroom Ganoderma multipileum using succinate dehydrogenase mutation gene as a selection marker

Fig. 2a,b

GmSdhBm gene structure and construction of the carboxin resistance vector. a pBSGmSdhBm vector. Amp Ampicillin resistance gene used for selection of plasmids in Escherichia coli; M point mutation changing a histidine to a leucine in the coding region; * point mutation to create a restriction site for PCR-RFLP; F and R represent forward and reverse primers used in PCR-RFLP. b GmSdhBm gene structure predicted by Augustus gene prediction software (http://bioinf.uni-greifswald.de/augustus/). Arrows Exons

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