Protoplasting, regeneration and transformation of medicinal mushroom Ganoderma multipileum using succinate dehydrogenase mutation gene as a selection marker

Table 1 Factors affect Ganoderma multipileum protoplasting

Factor	Different parameters
pH of lysing enzyme^a	5.0, 5.5, 5.8, 6.0
Lysing enzyme concentration (mg/mL)	5.0, 7.5, 10
Osmoticum	KCl, Mannitol, Sucrose, MgSO₄⋅7H₂O
Enzyme digestion time (h)	2, 3, 4, 5
Mycelium age (days)	4, 5, 6

^apH of the lysing enzyme solution: pH 5 was adjusted with sodium acetate buffer; pH 5.5, 5.8 and 6.0 were prepared with potassium phosphate buffer

ISSN: 1869-2044