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Table 3 Primers used in this G. multipileum study

From: Protoplasting, regeneration and transformation of medicinal mushroom Ganoderma multipileum using succinate dehydrogenase mutation gene as a selection marker

Primer name

Primer sequence 5′→3′

Purposes of primers

gmSdhB-Sense-0503

ACCTCCATTACCCAACGGAAACCAC

Construct

gmSdhB-Sense 812–0503

CTGGTCTGGTGAGGCTTGGATGAGG

Sequencing

gmSdhB-Sense 1490–0503

TTGACAGGAACGCTGGCAAGGAT

PCR-RFLP

gmSdhB-Sense 2127–0503

CGGATGGAGAAGATGCAGAATGAGC

Sequencing

gmSdhB-AS-0503

TTTATTGCACGTCGCCGGTGAGAAC

Construct, PCR-RFLP

gmSdhB-Sense-HIS mutation-0503

GAGCTCAGCCTGTACCGGTGCCTCAC

Point mutation switch His to Leu and creation of an AgeI restriction site