Phenotypic and genotypic evaluation of Listeria monocytogenes strains isolated from fish and fish processing plants

Table 1 Primer sequences and amplification conditions

Reaction	Virulence gene	Primer sequences (5′-3′)	Amplicon size [bp]	Reference	Amplification conditions
MIX I	fbpA	TTATTTCCTCGCATCCTAGC	435	This study	Initial denaturation—94 °C/2 min. 35 cycles: denaturation—94 °C/15 s, annealing—48.5 °C/30 s, elongation—72 °C/50 s final elongation—72 °C/1 min
	fbpA	TATCAATTCGACCTGCTGAG	435	This study
	plcA	ACACGAGCAATAAAATCCCT	278	Suo et al. (2010)
	plcA	ATACTGACGAGGTGTGAATG	278	Suo et al. (2010)
	hlyA	TTTTCGATTGGCGTCTTAGGA	101	This study
	hlyA	ACTGAAGCAAAGGATGCATCTG	101	This study
MIX II	plcB	GCAAGTGTTCTAGTCTTTCCGG	794	Franciosa et al. (2005)	Initial denaturation—94 °C/2 min 16 cycles: denaturation—94 °C/30 s, annealing—57 °C/45 s, elongation—72 °C/45 s 19 cycles: denaturation—94 °C/30 s, annealing—49 °C/45 s, elongation—72 °C/45 s Final elongation—72 °C/1 min
	plcB	ACCTGCCAAAGTTTGCTGTGA	794	Franciosa et al. (2005)
	inlB	TCCGACTAAACAAGGCTATG	302	this study
	inlB	TGTACCATAATTTTCCGCCA	302	this study
	actA	ACGAACAAAGCAGACCTAAT	231	this study
	actA	TGTACCATAATTTTCCGCCA	231	this study
	iap	ACAAGCTGCACCTGTTGCAG	131	Rawool et al. (2007)
	iap	TGACAGCGTGTGTAGTAGCA	131	Rawool et al. (2007)
MIX III	inlA	CAGGCAGCTACAATTACACA	2341	Franciosa et al. (2005)
	inlA	ATATAGTCCGAAAACCACATCT	2341
	mpl	TATGACGGTAAAAGCAGATT	1458
	mpl	TTCCCAAGCTTCAGCAACTT	1458
	prfA	CATGAACGCTCAAGCAGAAG	706
	prfA	AATTTTCCCAAGTAGCAGGA	706

ISSN: 1869-2044