Table 2 Phenotypic and genotypic characterization of L. monocytogenes strains tested (S-sensitive, R-resistant, P-penicillin, AM-ampicillin, MEM-meropenem, E-erythromycin, SXT-cotrimoxazole)
Strain number | Origin | Serogroup | Virulence genes | Drug resistance | Coaggregation level with S. enteritidis | |
|---|---|---|---|---|---|---|
Fish | 1 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 32.2 |
2 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 31.0 | |
3 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 27.6 | |
4 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 36.3 | |
5 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 29.8 | |
6 | Smoked fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 32.7 | |
7 | Smoked fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 35.1 | |
8 | Whole salmon; raw material | 1/2b–3b | hlyA, plcA, iap, plcB, inlB, actA, inlA, prfA | R: -; S: P, AM, MEM, E, SXT | 35.7 | |
11 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 35.1 | |
14 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 32.8 | |
15 | Fresh fillet | 4b–4d–4e | hlyA, plcA, iap, plcB, inlB, actA, inlA, prfA | R: -; S: P, AM, MEM, E, SXT | 35.7 | |
17 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 32.8 | |
19 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 32.8 | |
20 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, iap, plcB, inlB, actA, inlA, prfA | R: -; S: P, AM, MEM, E, SXT | 33.9 | |
21 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: P; S: AM, MEM, E, SXT | 32.8 | |
24 | Fresh fillet | 4b–4d–4e | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: E; S: P, AM, MEM, SXT | 24.6 | |
26 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 26.3 | |
28 | Whole salmon; raw material | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 19.3 | |
29 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: P; S: AM, MEM, E, SXT | 29.8 | |
33 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 32.8 | |
34 | Smoked fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: MEM, SXT; S: P,AM, E | 28.1 | |
35 | Smoked fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: MEM, E; S: P, AM, SXT | 32.2 | |
37 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 33.9 | |
38 | Fresh fillet | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 28.1 | |
40 | Whole salmon; raw material | 4b–4d–4e | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: P, AM, E; S: MEM, SXT | 35.1 | |
41 | Fresh fillet | 4b–4d–4e | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: P; S: AM, MEM, E, SXT | 35.1 | |
Production equipment at the fish processing plant | 10 | Skinning machine | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 33.9 |
12 | Guillotine | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 16.5 | |
16 | Brine | 4b–4d–4e | hlyA, plcA, iap, plcB, inlB, actA, inlA, prfA | R: -; S: P, AM, MEM, E, SXT | 35.7 | |
18 | Slicer conveyor rollers | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 29.2 | |
22 | Rolls of fish-bone remover | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: E; S: AM, MEM, E, SXT | 33.9 | |
23 | Slicer conveyor tape | 4b–4d–4e | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 22.8 | |
30 | Skinning machine | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 29.8 | |
31 | Skinning machine | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: P,AM, E; S: MEM, SXT | 25.9 | |
32 | Rolls of fish-bone remover | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: P; S: AM, MEM, E, SXT | 28.1 | |
36 | Injector needles | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: -; S: P, AM, MEM, E, SXT | 35.1 | |
39 | Brine | 1/2a–3a | hlyA, plcA, fbpA, iap, plcB, inlB, actA, inlA, mpl, prfA | R: SXT; S: P, AM, MEM, E | 35.1 |