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Table 2 Methods of detecting intracellular carbohydrate and bud scars of yeast cell

From: Serial re-pitching: its effect on yeast physiology, fermentation performance, and product quality

Biomarkers

Methods

Wave length

Output

Glycogen

Acriflavine staining

488 nm

Emits green fluorescence

Trehalose

Lectin-fluorochrome-conjugate concanavalin A–fluorescein

488 nm

Detects green fluorescence

Bud scars

Staining with WGA fluorescein dye

500–580 nm

Circular, bright yellow-green fluorescent on the cell surface: high fluorescent-many aged cells; low fluorescence- a young cell in the population

  1. Schlee et al. 2006; WGA, wheat germ agglutinin fluorescein