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Fig. 1 | Annals of Microbiology

Fig. 1

From: IgY antibodies of chicken do not bind staphylococcal binder of immunoglobulin (Sbi) from Staphylococcus aureus

Fig. 1

Heterologous cloning and expression of sbi gene of S. aureus in E.coli and its purification a. Agarose gel showing PCR amplified sbi gene using Pfu DNA polymerase, b. Coomassie stained SDS-PAGE gel showing expression of sbi gene after IPTG induction, c. Western blot probed with anti-6× histidine antibody showing expression of r-Sbi protein, d. Coomassie Brilliant Blue stained SDS-PAGE gel showing purified recombinant Sbi protein

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