Fig. 4

Recombinant protein enzyme of xynA, xynADCBM, and CBM binding to xylan and avicel substrate. a xynA and xynADCBM binding to xylan. 1–4, xynA binding to xylan. M, marker; 1, control protein; 2, supernatant (unbound protein); 3, precipitated eluent (nonspecific binding protein); 4, precipitated eluent after denaturation (substrate binding protein); 5–8, xynADCBM binding to xylan. 5, control protein; 6, supernatant (unbound protein); 7, precipitated eluent (nonspecific binding protein); 8, precipitated eluent after denaturation (substrate binding protein). b CBM binding to xylan. M, marker; 1, control protein; 2, supernatant (unbound protein); 3, precipitated eluent (nonspecific binding protein); 4, precipitated eluent after denaturation (substrate binding protein). c xynA binding to avicel. M, marker; 1, control protein; 2, supernatant (unbound protein); 3, precipitated eluent (nonspecific binding protein); 4, precipitated eluent after denaturation (substrate binding protein). d xynADCBM binding to avicel. M, marker; 1, control protein; 2, supernatant (unbound protein); 3, precipitated eluent (nonspecific binding protein); 4, precipitated eluent after denaturation (substrate binding protein). e CBM binding to avicel. M, marker; 1, control protein; 2, supernatant (unbound protein); 3, precipitated eluent (nonspecific binding protein); 4, precipitated eluent after denaturation (substrate binding protein)