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Table 2 qPCR parameters of standard curves for Bifidobacterium animalis subsp. lactis (Nu-trish®BB-12®) extracted from B. lactis grown in culture medium

From: Application of propidium monoazide coupled with quantitative PCR to evaluate cell viability of Bifidobacterium animalis subsp. lactis in a non-dairy probiotic beverage

qPCR run

Efficiency (%)

Slope

R2

1

81

− 3.890

0.9965

2

87

− 3.685

0.9997

3

87

− 3.689

0.9998

4

90

− 3.588

0.9994

5

88

− 3.638

0.9993

6

92

− 3.526

0.9991

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Annals of Microbiology

ISSN: 1869-2044

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