Application of propidium monoazide coupled with quantitative PCR to evaluate cell viability of Bifidobacterium animalis subsp. lactis in a non-dairy probiotic beverage

Table 3 Parameters of standard curves for Bifidobacterium animalis subsp. lactis (Nu-trish®BB-12®) quantification using tuf primers and bacterial DNA extracted from three formulations of microencapsulated probiotic passion fruit juice 1 day after microencapsulation process

qPCR run	Efficiency (%)	Slope	R²
M1 (20% maltodextrin)^*
1	94	− 3.464	0.9988
2	98	− 3.378	0.9983
3	88	− 3.642	0.9993
M2 (10% maltodextrin 10% inulin)^*
1	85	− 3.755	0.9997
2	92	− 3.520	0.9995
3	86	− 3.690	0.9983
M3 (20% inulin)^*
1	78	− 3.987	0.9957
2	80	− 3.920	0.9817
3	87	− 3.681	0.9978

ISSN: 1869-2044