Skip to main content
Fig. 5 | Annals of Microbiology

Fig. 5

From: Determination of steroid hydroxylation specificity of an industrial strain Aspergillus ochraceus TCCC41060 by cytochrome P450 gene CYP68J5

Fig. 5

TLC and HPLC analyses of biotransformation products. a TLC assays: lanes 1–4, EP as substrate, petroleum and ethyl acetate (3:2) as the solvent system; lanes 5–8, DE as substrate, petroleum and ethyl acetate (1:1) as the solvent system. 1, A. ochraceus TCCC41060; 2, the cyp68j5 mutant; 3, the reconstituted strain; 4, EP and 11α-OH EP standards; 5, DE and 11α-OH DE standards; 6, A. ochraceus TCCC41060; 7, the cyp68j5 mutant; 8, the reconstituted strain. b, c HPLC assays: acetonitrile: H2O 80:20 (v/v) as the mobile phase, b EP as substrate: EP standard (a), 11α-OH EP standard (b), transformation products of recipient strain (c), the cyp68j5 mutant (d), and the reconstituted strain (e); c DE as substrate: DE standard (a), 11α-OH DE standard (b), transformation products of the recipient strain (c), the cyp68j5 mutant (d), and the reconstituted strain (e)

Back to article page