Table 1 Effect of mono- and divalent metal ions, metal-chelating agents, surfactants, and phenolic compounds on CMCase activity (mean values of three replicates)
Agent | Concentration | Residual activity (%) |
|---|---|---|
None | Â | 100 |
Mn2+ (MnCl2·2 H2O) | 10 mM | 36 |
Fe3+ (FeCl3) | 10 mM | 75 |
Mg2+ (MgCl2·6 H2O) | 10 mM | 111 |
Pb2+ (Pb(NO3)2) | 10 mM | 56 |
Zn2+ (ZnSO4·7 H2O) | 10 mM | 111 |
Cu2+ (CuCl2·2 H2O) | 10 mM | 110 |
Fe2+ (FeCl2·4 H2O) | 10 mM | 11 |
K+ (KCl) | 100 mM | 101 |
Na+ (NaCl) | 100 mM | 111 |
EDTA (Titriplex III) | 3 % (w/v) | 109 |
Ethylene glycol | 3 % (v/v) | 103 |
Triton X-100 | 3 % (v/v) | 125 |
Tween 20 | 3 % (v/v) | 82 |
Phenol | 10 mM | 103 |