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Table 2 Steepest ascent experiment

From: High-level production of γ-cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21 (DE3): culture medium optimization, enzymatic properties characterization, and product specificity analysis

Run Variable CGTase activity (U mL−1)
Yeast extract (g L−1) MgSO4 (mmol L−1) NiSO4 (mmol L−1)
1 18 2.67 1.46 28094.42
2 27 3.33 1.23 32509.23
3 36 4 1 50778.34
4 45 4.67 0.77 42607.18
5 54 5.33 0.54 43044.62
6 63 6 0.31 40944.88
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Annals of Microbiology

ISSN: 1869-2044

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