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Table 2 Steepest ascent experiment

From: High-level production of γ-cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21 (DE3): culture medium optimization, enzymatic properties characterization, and product specificity analysis

Run

Variable

CGTase activity (U mL−1)

Yeast extract (g L−1)

MgSO4 (mmol L−1)

NiSO4 (mmol L−1)

1

18

2.67

1.46

28094.42

2

27

3.33

1.23

32509.23

3

36

4

1

50778.34

4

45

4.67

0.77

42607.18

5

54

5.33

0.54

43044.62

6

63

6

0.31

40944.88

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Annals of Microbiology

ISSN: 1869-2044

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