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Table 3 The BBD matrix for coded variables along with actual and predicted responses

From: High-level production of γ-cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21 (DE3): culture medium optimization, enzymatic properties characterization, and product specificity analysis

Run

A

B

C

CGTase activity (U mL−1)

Actual response

Predicted response

Residual

1

0

0

0

52591.46

53615.57

−1024.11

2

0

1

1

48695.64

48236.91

458.72

3

0

0

0

54168.75

53615.57

553.18

4

1

−1

0

47940.07

48009.29

−69.21

5

−1

0

−1

43689.00

43365.18

323.82

6

0

0

0

52820.12

53615.57

−795.46

7

−1

1

0

46464.77

46395.55

69.21

8

0

0

0

54618.22

53615.57

1002.65

9

0

1

−1

48267.38

48660.41

−393.04

10

−1

−1

0

47786.02

47651.12

134.90

11

0

−1

1

50046.18

49653.15

393.04

12

0

0

0

53879.31

53615.57

263.74

13

−1

0

1

44883.23

45411.17

−1007.58

14

0

−1

−1

43933.89

50002.88

−527.94

15

1

0

−1

46560.11

46032.17

1007.58

16

1

1

0

51981.52

61103.53

527.94

17

1

0

1

48499.38

48823.21

−323.82