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Table 4 ANOVA results of the quadratic model for CGTase activity (R2 = 0.9776; Adj R2 = 0.9487)

From: High-level production of γ-cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21 (DE3): culture medium optimization, enzymatic properties characterization, and product specificity analysis

Source

SS

df

MS

F-Value

p value

 

Model

2.008 × 108

9

2.231 × 107

33.91

< 0.0001

Significant

A

1.848 × 107

1

1.848 × 107

28.08

0.0011

 

B

4.066 × 106

1

4.066 × 106

6.18

0.0418

 

C

1.170 × 107

1

1.170 × 107

17.78

0.0040

 

AB

7.190 × 106

1

7.190 × 106

10.93

0.0130

 

AC

1.388 × 105

1

1.388 × 105

0.21

0.6600

 

BC

8.077 × 106

1

8.077 × 106

12.28

0.0099

 

A2

5.012 × 107

1

5.012 × 107

76.18

< 0.0001

 

B2

1.108 × 107

1

1.108 × 107

16.84

0.0045

 

C2

7.632 × 107

1

7.632 × 107

116.00

< 0.0001

 

Residual

4.605 × 106

7

6.579 × 105

   

Lack of fit

1.543 × 106

3

5.143 × 105

0.67

0.6126

Not significant

Pure error

3.062 × 106

4

7.656 × 105

   

Cor total

2.054 × 108

16

    
  1. SS sum of squares, df degrees of freedom, MS mean square