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Table 2 Experimental design for melanin production optimization by D. schimae NM3 (layout and results)

From: Biocompatibility and radioprotection by newly characterized melanin pigment and its production from Dietzia schimae NM3 in optimized whey medium by response surface methodology

Std

Run

Factor 1

Factor 2

Factor 3

Factor 4

Response 1

A: pH

B: temperature, °C

C: L-tyrosine, g l−1

D: CuSO4, g l−1

Melanin yield, mg l−1

2

1

12.0

29

2.5

0.013

140

24

2

10.5

35

2.5

0.020

550

17

3

9.0

32

1

0.013

280

10

4

12.0

32

2.5

0.005

80

26

5

10.5

32

2.5

0.013

790

11

6

9.0

32

2.5

0.020

300

5

7

10.5

32

1

0.005

280

6

8

10.5

32

4

0.005

300

21

9

10.5

29

2.5

0.005

220

15

10

10.5

29

4

0.013

350

18

11

12.0

32

1

0.013

80

19

12

9.0

32

4

0.013

320

23

13

10.5

29

2.5

0.020

320

16

14

10.5

35

4

0.013

600

4

15

12.0

35

2.5

0.013

290

3

16

9.0

35

2.5

0.013

280

20

17

12.0

32

4

0.013

310

27

18

10.5

32

2.5

0.013

770

13

19

10.5

29

1

0.013

320

22

20

10.5

35

2.5

0.005

260

14

21

10.5

35

1

0.013

400

9

22

9.0

32

2.5

0.005

220

25

23

10.5

32

2.5

0.013

780

7

24

10.5

32

1

0.020

400

1

25

9.0

29

2.5

0.013

250

12

26

12.0

32

2.5

0.020

280

8

27

10.5

32

4

0.020

550