Table 3 Analyzing variance (ANOVA) and regression for the fitted quadratic model of melanin production
Source | Sum of squares | Df | Mean square | F-value | p-value | Sig. |
|---|---|---|---|---|---|---|
Model | 9.635E+ 05 | 14 | 68,823.21 | 78.84 | < 0.0001 | Significant |
A: pH | 18,408.33 | 1 | 18,408.33 | 21.09 | 0.0006 | |
B: temperature | 50,700.00 | 1 | 50,700.00 | 58.08 | < 0.0001 | |
C: L-tyrosine | 37,408.33 | 1 | 37,408.33 | 42.85 | < 0.0001 | |
D: CuSO4 | 90,133.33 | 1 | 90,133.33 | 103.26 | < 0.0001 | |
AB | 3600.00 | 1 | 3600.00 | 4.12 | 0.0650 | |
AC | 9025.00 | 1 | 9025.00 | 10.34 | 0.0074 | |
AD | 3600.00 | 1 | 3600.00 | 4.12 | 0.0650 | |
BC | 7225.00 | 1 | 7225.00 | 8.28 | 0.0139 | |
BD | 9025.00 | 1 | 9025.00 | 10.34 | 0.0074 | |
CD | 4225.00 | 1 | 4225.00 | 4.84 | 0.0481 | |
A2 | 6.302E+ 05 | 1 | 6.302E+ 05 | 721.96 | < 0.0001 | |
B2 | 2.133E+ 05 | 1 | 2.133E+ 05 | 244.39 | < 0.0001 | |
C2 | 1.610E+ 05 | 1 | 1.610E+ 05 | 184.45 | < 0.0001 | |
D2 | 2.760E+ 05 | 1 | 2.760E+ 05 | 316.22 | < 0.0001 | |
Residual | 10,475.00 | 12 | 872.92 | |||
Lack of Fit | 10,275.00 | 10 | 1027.50 | 10.27 | 0.0919 | Not significant |
Pure Error | 200.00 | 2 | 100.00 | |||
Corrected total | 9.740E+ 05 | 26 |