Target genes | Initial denaturation | Amplification (35 cycles) | Final extension | ||
---|---|---|---|---|---|
Denaturation | Annealing | Extension | |||
mecA | 94 °C/1.5 min | 95 °C for 45 s | 55 °C for 1 min | 72 °C/45 s | 72 °C/10 min |
16SrRNA S. aureus specific | 94 °C/5 min | 94 °C/30 s | 55 °C/30 s | 72 °C/45 s | 72 °C/5 min |
sea | 95 °C/10 min | 94 °C/2 min | 55 °C/2 min | 72 °C/1 min | 72 °C/1 min |
tsst-1 | 95 °C/10 min | 94 °C/2 min | 55 °C/2 min | 72 °C/1 min | 72 °C/1 min |
eta | 94 °C/5 min | 94 °C/30 s | 57 °C/30 s | 72 °C/45 s | 72 °C/10 min |
hlb | 94 °C/5 min | 94 °C/30 s | 58 °C/30 s | 72 °C/20 s | 72 °C/10 min |
clfA | 94 °C/10 min | 94 °C/10 min | 55 °C/1 min | 72 °C/1 min | 72 °C/10 min |
icaD | 94 °C/10 min | 94 °C/30 s | 53 °C/30 s | 72 °C/30 s | 72 °C/10 min |