Molecular characterization of virulence factors in Staphylococcus aureus isolated from bovine subclinical mastitis in central Ethiopia

Table 2 Cyclic polymerase chain reaction conditions of the different primer sets

Target genes	Initial denaturation	Amplification (35 cycles)			Final extension
Target genes	Initial denaturation	Denaturation	Annealing	Extension	Final extension
mecA	94 °C/1.5 min	95 °C for 45 s	55 °C for 1 min	72 °C/45 s	72 °C/10 min
16SrRNA S. aureus specific	94 °C/5 min	94 °C/30 s	55 °C/30 s	72 °C/45 s	72 °C/5 min
sea	95 °C/10 min	94 °C/2 min	55 °C/2 min	72 °C/1 min	72 °C/1 min
tsst-1	95 °C/10 min	94 °C/2 min	55 °C/2 min	72 °C/1 min	72 °C/1 min
eta	94 °C/5 min	94 °C/30 s	57 °C/30 s	72 °C/45 s	72 °C/10 min
hlb	94 °C/5 min	94 °C/30 s	58 °C/30 s	72 °C/20 s	72 °C/10 min
clfA	94 °C/10 min	94 °C/10 min	55 °C/1 min	72 °C/1 min	72 °C/10 min
icaD	94 °C/10 min	94 °C/30 s	53 °C/30 s	72 °C/30 s	72 °C/10 min

ISSN: 1869-2044