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Table 6 Enzyme characterization and carbohydrate utilization of selected LAB

From: Aggrandizement of fermented cucumber through the action of autochthonous probiotic cum starter strains of Lactiplantibacillus plantarum and Pediococcus pentosaceus

   NPL 1258 NPL 1259 NPL 1264 NPL 1277 NPL 1279 NPL 1280 NPL 1284 NPL 1286 NPL 1291 NPL 1306 ATCC 8014
Sr. no. Carbohydrate fermentation            
1 Lactose + + + + + + + + + + +
2 Xylose + + + + + + + + + + +
3 Maltose + + - + + + + + - + +
4 Fructose + - + + - + + + + + +
5 Dextrose + - + + - + + - + + +
6 Galactose + - + + - + + - + + +
7 Raffinose + - + + - + + + + - -
8 Trehalose + + + + + + + + + + +
9 Melibiose - - - - - - - - - - -
10 Sucrose + - + + - + + + + + +
11 L-Arabinose + + + + + + + + + + +
12 Mannose + - + + - + + + + + +
13 Inulin + + + + + + + + + + +
14 Sodium gluconate - - - - - - - - - - -
15 Glycerol - - - - - + + - - - -
16 Salicin + + + + + + + + + - +
17 Dulcitol - - - - - - + - - - -
18 Inositol - - - - - - - - - - -
19 Sorbitol + + + + + + + + + + +
20 Mannitol + + + + + + + + + + +
21 Adonitol - - - - - - + - - - +
22 Arabitol - - - - - - + - - + +
23 Erythritol - - - - - - - - - - -
24 α-Methyl-D glucoside - - - - - - + - - - -
25 Rhamnose - - - - - - + - - - -
26 Cellobiose + + + + + + + + + + +
27 Melezitose - + - - - - + - - - -
28 α-methyl-D mannoside + + - - - - + - - - -
29 Xylitol + + - - - - + - - - -
30 ONPG + + - - - - + - - - -
31 Esculin hydrolysis + + + + + + + + + + +
32 D-Arabinose + + + + + + + + + + +
33 Citrate utilization - + - - - + + + - - +
34 Malonate utilization - + - - - - - - - - -
35 Sorbose - - - - - - - - - - -
Sr. no. Enzyme activity            
1 Alkaline phosphatase 0 0 0 0 0 0 0 0 0 0 0
2 Esterase (C 4) 0 0 0 0 0 0 0 0 0 0 0
3 Esterase lipase (C 8) 5 5 10 5 10 10 10 5 10 5 5
4 Lipase (C 14) 0 0 0 0 0 0 0 0 0 0 0
5 Leucine arylamidase > 40 20 20 20 > 40 > 40 > 40 20 10 10 > 40
6 Valine arylamidase 20 20 20 20 20 20 20 20 5 5 10
7 Cystine arylamidase > 40 > 40 20 20 20 20 20 20 20 20 20
8 Trypsin 0 0 0 0 0 0 0 0 0 0 0
9 α-chymotrypsin 0 0 0 0 0 0 0 0 0 0 0
10 Acid phosphatase 5 5 5 5 5 5 5 5 5 5 10
11 Naphthol-AS-BI-phosphohydrolase 5 5 5 5 5 5 5 5 5 5 20
12 α-galactosidase 0 0 0 0 0 0 0 0 0 0 0
13 ß-galactosidase 20 20 > 40 20 20 > 40 20 20 20 20 20
14 ß-glucuronidase 0 0 0 0 0 0 0 0 0 0 0
15 α-glucosidase 0 0 0 0 0 0 0 0 0 0 0
16 ß-glucosidase 20 10 20 10 5 5 5 10 5 10 20
17 N-acetyl-ß-glucosaminidase 0 0 0 0 0 0 0 0 0 0 0
18 α-mannosidase 0 0 0 0 0 0 0 0 0 0 0
19 α-fucosidase 0 0 0 0 0 0 0 0 0 0 0
  1. Enzyme activities were assessed through API-ZYM galleries that were expressed in terms of color intensity, which ranged from 0 (no activity), 5–10 (low), 30 (moderate), and ≥ 40 nmol (strong) of substrate hydrolyzed following 4 h incubation at 37 °C. Color intensity was judged with reference to an API ZYM color chart provided by the manufacturer. ‘+’ shows a positive reaction; ‘−’ shows a negative reaction