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Table 6 Enzyme characterization and carbohydrate utilization of selected LAB

From: Aggrandizement of fermented cucumber through the action of autochthonous probiotic cum starter strains of Lactiplantibacillus plantarum and Pediococcus pentosaceus

  

NPL 1258

NPL 1259

NPL 1264

NPL 1277

NPL 1279

NPL 1280

NPL 1284

NPL 1286

NPL 1291

NPL 1306

ATCC 8014

Sr. no.

Carbohydrate fermentation

           

1

Lactose

+

+

+

+

+

+

+

+

+

+

+

2

Xylose

+

+

+

+

+

+

+

+

+

+

+

3

Maltose

+

+

-

+

+

+

+

+

-

+

+

4

Fructose

+

-

+

+

-

+

+

+

+

+

+

5

Dextrose

+

-

+

+

-

+

+

-

+

+

+

6

Galactose

+

-

+

+

-

+

+

-

+

+

+

7

Raffinose

+

-

+

+

-

+

+

+

+

-

-

8

Trehalose

+

+

+

+

+

+

+

+

+

+

+

9

Melibiose

-

-

-

-

-

-

-

-

-

-

-

10

Sucrose

+

-

+

+

-

+

+

+

+

+

+

11

L-Arabinose

+

+

+

+

+

+

+

+

+

+

+

12

Mannose

+

-

+

+

-

+

+

+

+

+

+

13

Inulin

+

+

+

+

+

+

+

+

+

+

+

14

Sodium gluconate

-

-

-

-

-

-

-

-

-

-

-

15

Glycerol

-

-

-

-

-

+

+

-

-

-

-

16

Salicin

+

+

+

+

+

+

+

+

+

-

+

17

Dulcitol

-

-

-

-

-

-

+

-

-

-

-

18

Inositol

-

-

-

-

-

-

-

-

-

-

-

19

Sorbitol

+

+

+

+

+

+

+

+

+

+

+

20

Mannitol

+

+

+

+

+

+

+

+

+

+

+

21

Adonitol

-

-

-

-

-

-

+

-

-

-

+

22

Arabitol

-

-

-

-

-

-

+

-

-

+

+

23

Erythritol

-

-

-

-

-

-

-

-

-

-

-

24

α-Methyl-D glucoside

-

-

-

-

-

-

+

-

-

-

-

25

Rhamnose

-

-

-

-

-

-

+

-

-

-

-

26

Cellobiose

+

+

+

+

+

+

+

+

+

+

+

27

Melezitose

-

+

-

-

-

-

+

-

-

-

-

28

α-methyl-D mannoside

+

+

-

-

-

-

+

-

-

-

-

29

Xylitol

+

+

-

-

-

-

+

-

-

-

-

30

ONPG

+

+

-

-

-

-

+

-

-

-

-

31

Esculin hydrolysis

+

+

+

+

+

+

+

+

+

+

+

32

D-Arabinose

+

+

+

+

+

+

+

+

+

+

+

33

Citrate utilization

-

+

-

-

-

+

+

+

-

-

+

34

Malonate utilization

-

+

-

-

-

-

-

-

-

-

-

35

Sorbose

-

-

-

-

-

-

-

-

-

-

-

Sr. no.

Enzyme activity

           

1

Alkaline phosphatase

0

0

0

0

0

0

0

0

0

0

0

2

Esterase (C 4)

0

0

0

0

0

0

0

0

0

0

0

3

Esterase lipase (C 8)

5

5

10

5

10

10

10

5

10

5

5

4

Lipase (C 14)

0

0

0

0

0

0

0

0

0

0

0

5

Leucine arylamidase

> 40

20

20

20

> 40

> 40

> 40

20

10

10

> 40

6

Valine arylamidase

20

20

20

20

20

20

20

20

5

5

10

7

Cystine arylamidase

> 40

> 40

20

20

20

20

20

20

20

20

20

8

Trypsin

0

0

0

0

0

0

0

0

0

0

0

9

α-chymotrypsin

0

0

0

0

0

0

0

0

0

0

0

10

Acid phosphatase

5

5

5

5

5

5

5

5

5

5

10

11

Naphthol-AS-BI-phosphohydrolase

5

5

5

5

5

5

5

5

5

5

20

12

α-galactosidase

0

0

0

0

0

0

0

0

0

0

0

13

ß-galactosidase

20

20

> 40

20

20

> 40

20

20

20

20

20

14

ß-glucuronidase

0

0

0

0

0

0

0

0

0

0

0

15

α-glucosidase

0

0

0

0

0

0

0

0

0

0

0

16

ß-glucosidase

20

10

20

10

5

5

5

10

5

10

20

17

N-acetyl-ß-glucosaminidase

0

0

0

0

0

0

0

0

0

0

0

18

α-mannosidase

0

0

0

0

0

0

0

0

0

0

0

19

α-fucosidase

0

0

0

0

0

0

0

0

0

0

0

  1. Enzyme activities were assessed through API-ZYM galleries that were expressed in terms of color intensity, which ranged from 0 (no activity), 5–10 (low), 30 (moderate), and ≥ 40 nmol (strong) of substrate hydrolyzed following 4 h incubation at 37 °C. Color intensity was judged with reference to an API ZYM color chart provided by the manufacturer. ‘+’ shows a positive reaction; ‘−’ shows a negative reaction