Table 1 Primers used qPCR
Specificity | Primer | Sequence | Reference |
|---|---|---|---|
Gammaproteobacteria | Gam877F | 5′-GCTAACGCATTAAGTRYCCCG-3′ | Yang et al. 2015 |
Gam1066R | 5′-GCCATGCRGCACCTGTCT-3′ | ||
Firmicutes | Lgc353F | 5′-GCAGTAGGGAATCTTCCG-3′ | Fierer et al. 2005 |
Eub518R | 5′-ATTACCGCGGCTGCTGG-3′ | ||
Acidobacteria | Acid31 | 5′-GATCCTGGCTCAGAATC-3′ | Fierer et al. 2005 |
Eub518R | 5′-ATTACCGCGGCTGCTGG-3′ | ||
Trichoderma | uTr | 5′-AAGTTCAGCGGGTATTCCT-3′ | Fierer et al. 2005 |
uTf | 5′- AACGTTACCAAACTGTTG-3′ |