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Table 2 Results of the experiments at the 3 dilutions of viable, VBNC and non-viable cells with EMA at different concentrations (μg/ml)

From: Assessing the viability of Legionella pneumophila in environmental samples: regarding the filter application of Ethidium Monoazide Bromide

Dilutions at different EMA concentrations

Viable LogGU/L

VBNC LogGU/L

Non-viableLogGU/L

 

1^ qPCR (without EMA)

10.49

10.38

9.18

p = 0.20

1^ EMA 2.5 μg/ml

10.15

10.31

10.38

p = 0.16

1^ EMA 10 μg/ml

10.46

9.54

9.94

p = 0.43

1^ EMA 20 μg/ml

8.56

8.49

9.60

p = 0.84

 

p = 0.22

p = 0.10

p = 0.72

 

2^ qPCR (without EMA)

9.11

8.72

8.97

p = 0.60

2^ EMA 2.5 μg/ml

8.78

8.55

8.85

p = 0.76

2^ EMA 10 μg/ml

8.56

8.49

0.00

p = 0.22

2^ EMA 20 μg/ml

8.84

8.53

8.15

p = 0.16

 

p = 0.30

p = 0.17

p = 0.56

 

3^ qPCR (without EMA)

8.44

8.16

7.20

p = 0.18

3^ EMA 2.5 μg/ml

8.24

7.85

0.00

p = 0.21

3^ EMA 10 μg/ml

8.07

7.87

0.00

p = 0.22

3^ EMA 20 μg/ml

8.21

7.79

7.76

p = 0.21

 

p = 0.20

p = 0.14

p = 0.93