Table 4 The purification step of cellulase isolate from Klebsiella oxytoca (M21WG) and Klebsiella sp. (Z6WG)
Enzyme code | Purification steps | Total protein (mg) | Total activity (U) | Specific activity \(\frac{\left(\mathbf{U}\right)}{\left(\mathbf{mg}\right)}\) |
|---|---|---|---|---|
Klebsiella oxytoca M21WG | Crude enzymes | 815 | 270 | 0.33 ± 0.3 |
Ammonium sulfate precipitation | 18 | 134.2 | 7.45 ± 0.45 | |
Dialysis | 7.3 | 103 | 14.12 ± 0.58 | |
Klebsiella sp. Z6WG | Crude enzymes | 726 | 236.67 | 0.32 ± 0.74 |
Ammonium sulfate precipitation | 30.8 | 110 | 3.57 ± 0.53 | |
Dialysis | 7 | 95.3 | 13.61 ± 0.69 |