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Fig. 3 | Annals of Microbiology

Fig. 3

From: Leveraging quorum sensing system for automatic coordination of Escherichia coli growth and lactic acid biosynthesis

Fig. 3

Shake-flask cultivation of the recombinant E. coli strains. A Comparison of LdhA specific activities in two different mode strains that Q12 and Q15. B Cell growth in shake flask of strains. C Glucose consumption in shake flask. D Gene transcription of the strain overexpressing ldhA by “ON” mode. ldhA, D-lactate dehydrogenase coding gene; gapA and gapC, GAPDH coding genes; ptsH, PTS phosphocarrier protein coding genes; ptsI, PTS enzyme I coding gene; pykF, pyruvate kinase coding gene. E Comparison of gene transcription in Q12 upon 12 h and 22 h seed culture. F D-lactic acid. G Acetic acid. All data are presented as mean ± s.d. from three independent experiments. The statistical analysis is based on Mann-Whitney U test. *P < 0.05 and **P < 0.01

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