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Cloning and expression analysis of a predicted toxin gene fromPhotorhabdus sp. HB78
Annals of Microbiology volume 57, pages 313–319 (2007)
Abstract
The genome of the insect pathogenPhotorhabdus luminescens TT01 strain contains multiple genes predicted to encode toxins. One of these,plu0840, has 55% sequence identity with an enterotoxin fromAeromonas hydrophila. In order to further investigate this gene, we successfully cloned the completeplu0840 fromPhotorhabdus sp. HB78 and expressed it as a GST-Plu0840 fusion protein inEscherichia coli BL21 (DE3) using pGEX-4T-1 as a vector. Most of GST-Plu0840 was insoluble and sequestered into inclusion bodies. The inclusion bodies were harvested and dissolved. The resultant protein was cleaved and purified from the GST-tag. Oral bioassay showed that Plu0840 inhibited growth ofSpodoptera litura andSpodoptera exigua larvae.
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Li, M., Qiu, L. & Pang, Y. Cloning and expression analysis of a predicted toxin gene fromPhotorhabdus sp. HB78. Ann. Microbiol. 57, 313–319 (2007). https://doi.org/10.1007/BF03175066
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DOI: https://doi.org/10.1007/BF03175066