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  • Ecological and Environmental Microbiology
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Yeasts colonizing the leaves of fruit trees

Annals of Microbiology volume 59, pages 419–424 (2009)Cite this article

Abstract

Yeasts were isolated from leaf surfaces of five species of fruit trees located in southwest Slovakia. One hundred and fifty five yeast strains belonging to 11 genera were isolated from 300 samples of leaves. Seventeen yeast species were identified, but only three occurred regularly:Aureobasidium pullulans, Cryptococcus laurentii, andMetschnikowia pulcherrima. Species such asHanseniaspora uvarum, Pichia anomala, Rhodotorula glutinis, andSaccharomyces cerevisiae, were isolated less frequently. We found only few differences in the yeast community isolated from leaves of different tree species although dominant species occurred regularly on the majority of leaves. Furthermore, yeast species varied throughout years. In spite of the fact that the yeast community occupying the leaves of the fruit trees was studied in the samples harvested in three localities, which are distanced from each other some kilometers, the qualitative representation of the most isolated yeasts was identical. The differences were only in the frequency of the incidence of individual species in the samples.

References

  • Allen T.W., Burpee L.L., Buck J.W. (2004).In vitro attachment of phylloplane yeasts toBotrytis cinerea, Rhizoctonia solani, andSclerotinia homoeocarpa. Can. J. Microbiol., 50: 1041–1048.

    Article  CAS  PubMed  Google Scholar 

  • Andrews J.H., Harris R.F. (2000). The ecology and biogeography of microorganisms on plant surfaces. Annu. Rev. Phytopathol., 38: 1–36.

    Article  Google Scholar 

  • Andrews J.H., Spear R.N., Nordheim E.V. (2002). Population biology ofAureobasidium pullulans on apple leaf surfaces. Can. J. Microbiol., 48: 500–513.

    Article  CAS  PubMed  Google Scholar 

  • Barnett J.A., Payne R.W., Yarrow D. (2000). Yeasts: Characteristics and Identification. Third Edition, Cambridge University Press.

  • Blakeman J.P. (1973). The chemical environment of leaf surfaces with special reference to spore germination of pathogenic fungi. Pestic. Sci., 4: 575–588.

    Article  CAS  Google Scholar 

  • Camatti-Sartori V., da Silva-Ribeiro R.T., Valdebenito-Sanhueza R.M., Pagnocca F. C., Echeverrigaray S., Azevedo J.L. (2005). Endophytic yeasts and filamentous fungi associated with southern Brazilian apple (Malus domestica) orchards subjected to conventional, integrated or organic cultivation. J. Basic Microbiol., 45: 397–402.

    Article  PubMed  Google Scholar 

  • Comitini F., Ciani M. (2008). Influence of fungicide treatments on the occurrence of yeast flora associated with wine grapes. Ann. Microbiol., 58: 489–493.

    Article  Google Scholar 

  • Do Carmo-Sousa L. (1969). Distribution of yeasts in nature. In: Rose A.H., Harrison J.S., Eds, The Yeasts, Vol. I, Academic Press, London, pp. 79–105.

    Google Scholar 

  • Esteve-Zarzoso B., Belloch C., Uruburu F., Qerol A. (1999). Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int. J. Syst. Bacteriol., 49: 329–337.

    CAS  PubMed  Google Scholar 

  • Fiss M., Barckhausen O., Gherbawy Y., Kollar A., Hamamoto M., Auling G. (2003). Characterization of epiphytic yeasts of apple as potential biocontrol agents against apple scab (Venturia inaequalis). J. Plant Dis. Protect., 110: 513–523.

    Google Scholar 

  • Fleet G.H. (1998). Yeasts in natural habitats. Food Technol. Biotechnol., 36: 285–289.

    Google Scholar 

  • Fokkema N.J., den Houter J.G., Kosterman Y.J.C., Nelis A.L. (1979). Manipulation of yeasts on field-grown wheat leaves and their antagonistic effect onCochliobolus sativus andSeptoria nodorum. Trans. Br. Mycol. Soc., 72: 19–29.

    Article  Google Scholar 

  • Fredlund E., Druvefors U., Boysen M.E., Lingstein K.-J., Schnürer J. (2002). Physiological characteristics of the biocontrol yeastPichia anomala J121. FEMS Yeast Res., 2: 395–402.

    CAS  PubMed  Google Scholar 

  • Gadanho M., Sampaio J.P. (2002). Polyphasic taxonomy of the basidiomycetous yeast genusRhodotorula: Rh. glutinis sensu stricto andRh. dairensis comb. nov. FEMS Yeast Res., 2: 47–58.

    CAS  PubMed  Google Scholar 

  • Inácio J., Pereira P., De Carvalho M., Fonseca Á., Amaral-Collaco M.T., Spencer-Martins I. (2002). Estimation and diversity of phylloplane mycobiota on selected plants in a Mediterranean-type ecosystem in Portugal. Microb. Ecol., 44: 344–353.

    Article  PubMed  Google Scholar 

  • Ippolito A., Nigro F. (2000). Impact of preharvest application of biological control agents on postharvest diseases of fresh fruits and vegetables. Crop Protection, 19: 715–723.

    Article  Google Scholar 

  • Jager E., Wehner F., Korsten L. (2001). Microbial ecology of the mango phylloplane. Microb. Ecol., 42: 201–207.

    PubMed  Google Scholar 

  • Kurtzmann C.P., Fell J.W., Eds (1998). The Yeasts, A Taxonomic Study, 4th edn., Elsevier Science B.V., Amsterdam.

    Google Scholar 

  • Kvasnikov E.I., Nagornaia S.S., Shchelokova I.F. (1975). Yeast flora of plant rhizosphere and phyllosphere. Mikrobiologiia, 44: 339–346.

    CAS  PubMed  Google Scholar 

  • Lachance M.A. (1990). Yeast selection in nature. In: Panchal Ch.J., Ed., Yeast Strain Selection, Vol. 8, M. Dekker, New York, pp. 2–41.

    Google Scholar 

  • Lachance M.A., Starmer W.T., Rosa C.A., Bowles J.M., Barker J.S., Janzen D.H. (2001). Biogeography of the yeasts of ephemeral flowers and their insects. FEMS Yeast Res, 1: 1–8.

    CAS  PubMed  Google Scholar 

  • Leaw S.N., Chang H.C., Sun H.F., Barton R., Bouchara J.P., Chang T.Ch. (2006). Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions. J. Clin. Microbiol., 44: 693–699.

    Article  CAS  PubMed  Google Scholar 

  • Lima G., Curtis F.D., Castoria R., Cicco V.D. (1998). Activity of the yeastsCryptococcus laurentii andRhodotorula glutinis against post-harvest rots on different fruits. Biocontrol Sci. Technol., 8: 257–267.

    Article  Google Scholar 

  • Lindow S.E., Brandl M.T. (2003). Microbiology of the phyllosphere. Appl. Environ. Microbiol., 69: 1875–1883.

    Article  CAS  PubMed  Google Scholar 

  • McCormack P., Wildman H.G., Jeffries P. (1994). Production of antibacterial compounds by phylloplane-inhabiting yeasts and yeast like fungi. Appl. Environ. Microbiol., 60: 927–931.

    CAS  PubMed  Google Scholar 

  • Middelhoven W.J. (1997). Identity and biodegradative abilities of yeasts isolated from plants growing in an arid climate. Antoine van Leeuwenhoek, 72: 81–89.

    Article  CAS  Google Scholar 

  • Nakase T. (2000). Expanding world of ballistosporous yeasts: distribution in the phyllosphere, systematics and phylogeny. J. Gen. Appl. Microbiol., 46: 189–216.

    Article  PubMed  Google Scholar 

  • Neinhuis C., Barthlott W. (1998). Leaf surface contamination in beech, oak, and ginkgo in relation to micromorphology and wettability. New Phytol., 138: 91–98.

    Article  Google Scholar 

  • Pereira P.T., de Carvalho M.M., Girio F.M., Roseiro J.C., Amaral-Collaço M.T. (2002). Diversity of microfungi in the phylloplane of plants growing in a Mediterranean ecosystem. J. Basic Microbiol., 42: 396–407.

    Article  PubMed  Google Scholar 

  • Phaff H.J., Starmer W.T. (1987). Yeasts associated with plants, insects and soil. In: Rose A.H., Harrison J.S., Eds, The Yeasts, 2nd edn., Vol. 1, Academic Press London, pp. 123–180.

    Google Scholar 

  • Pimenta R.S., Morais P.B., Rosa C.A., Corrêa Jr.A. (2009). Utilization of yeasts in biological control programs. In: Satyanarayana T. and Kunze G., Eds, Yeast Biotechnology: Diversity and Applications, Springer, Netherlands, pp. 199–214.

    Chapter  Google Scholar 

  • Ribeiro I.C., Veríssimo I., Moniz L., Cardoso H., Sousa M.J., Soares A.M.V.M., Leão C. (2000). Yeasts as a model for assessing the toxicity of the fungicides Penconazol, Cymoxanil and Dichlofluanid. Chemosphere, 41: 1637–1642.

    Article  CAS  PubMed  Google Scholar 

  • Robinson T. (1974). Metabolism and function of alcaloids in plants. Science, 184: 430–435.

    Article  CAS  PubMed  Google Scholar 

  • Seibold A., Fried A., Kunz S., Moltmann E., Lange E., Jelkmann W. (2004). Yeasts as antagonists against fireblight. Bulletin OEPP/EPPO Bulletin, 34: 389–390.

    Google Scholar 

  • Sláviková E., Vadkertiová R. (1995). Yeasts and yeast-like organisms isolated from fish-pond waters. Acta Microbiol. Polonica, 44: 181–189.

    Google Scholar 

  • Sláviková E., Vadkertiová R., Vránová D. (2007). Yeasts colonizing the leaf surfaces. J. Basic Microbiol., 47: 344–350.

    Article  PubMed  CAS  Google Scholar 

  • Spencer J.F.T., Gorin P.A.J., Gardner N.R. (1974). Yeasts occurring in the effluent disposal basins of a pulp mill in Saskatchewan. Can. J. Microbiol., 20: 993–998.

    Article  CAS  PubMed  Google Scholar 

  • Sugita T., Takashima M., Ikeda R., Nakase T., Shinoda T. (2000). Intraspecies diversity ofCryptococcus laurentii as revealed by sequences of internal transcribed spacer regions and 28S rRNA gene and taxonomic position ofC. Laurentii clinical isolates. J. Clin. Microbiol., 38: 1468–1471.

    CAS  PubMed  Google Scholar 

  • Takashima M., Sugita T., Shinoda T., Nakase, T. (2003). Three new combinations from theCryptococcus laurentii complex:Cryptococcus aureus, Cryptococcus carnescens and Cryptococcus peneaus. Int. J. Syst. Evol. Microbiol., 53: 1187–1194.

    Article  CAS  PubMed  Google Scholar 

  • Yarrow D. (1998). Methods for isolation, maintenance and identification of yeasts. In: Kurtzman C.P., Fell J.W., Eds, The Yeasts, A Taxonomic Study, 4rd edn., Elsevier Science B.V., Amsterdam, pp. 77–100.

    Google Scholar 

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Authors and Affiliations

  1. Culture Collection of Yeasts, Institute of Chemistry Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia

    Elena SlÁviková & Renata Vadkertiová

  2. Institute of Food Science and Technology, Faculty of Chemistry, BUT, Purkyňova118, 612 00, Brno, Czech Republic

    Dana Vránová

Authors
  1. Elena SlÁviková
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  2. Renata Vadkertiová
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  3. Dana Vránová
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Correspondence to Elena SlÁviková.

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SlÁviková, E., Vadkertiová, R. & Vránová, D. Yeasts colonizing the leaves of fruit trees. Ann. Microbiol. 59, 419–424 (2009). https://doi.org/10.1007/BF03175125

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Annals of Microbiology

ISSN: 1869-2044

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