- Industrial Microbiology
- Original Articles
- Published:
Cellulase production by solid state fermentation using bagasse withPenicillium decumbens L-06
Annals of Microbiology volume 59, pages 517–523 (2009)
Abstract
The cellulase production byPenicillium decumbens L-06 in solid state fermentation (SSF) was investigated using bagasse as the substrate in this paper. The optimum conditions for cellulase production achieved by single factor testing were: the ratio of bagasse to wheat bran 1∶1 (w/w), the ratio of water to material 3∶1 (v/w), culture temperature 30 °C, initial pH 5.0, ammonium sulphate as nitrogen source with the concentration of 1%, 6 day’s fermentation period. BoxuBehnken factorial design (BBD) and response surface methodology (RSM) were further used to optimize conditions for cellulase (Filter paper activity) production. The maximal cellulase (Filter paper activity) production (3.89 FPu g−1) was obtained under the optimized conditions (ratio of water to material 2.38∶1, initial pH 5.28, cultivation time 150.5 h). It was well corresponded to the calculated results (3.97 FPu g−1) by model prediction.
References
Ghose T.K. (1987). Measurement of cellulase activities. Pure Appl. Chem., 59: 257–268.
Haddadin M.S.Y., Abu-Reesh I.M., Haddadin F.A.S., Robinson R.K. (2001). Utilisation of tomato pomace as a substrate for the production of vitamin B12 — a preliminary appraisal. Bioresour. Technol., 78: 225–230.
Kang S.W., Park Y.S., Lee J.S., Hong S.I., Kim S.W. (2004). Production of cellulases and hemicellulases byAspergillus niger KK2 from lignocellulosic biomass. Bioresour. Technol., 91, 153–156.
Kapdan I.K., Kargi F. (2006). Bio-hydrogen production from waste materials. Enzyme Microb. Technol., 38 (5): 569–582
Krishna C. (2005). Solid-state fermentation systems: An overview. Crit. Rev. Biotechnol., 25: 1–30.
Kumar N., Das D. (2001). Continuous hydrogen production by immobilizedEnterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. Enzyme Microb. Technol., 29 (4–5): 280–287.
Latifian M., Hamidi-Esfahani Z., Barzegar M. (2007). Evaluation of culture conditions for cellulase production by twoTrichoderma reesei mutants under solid-state fermentation conditions. Bioresour. Technol., 98: 3634–3637.
Li Y., Cui F.J., Liu Z.Q., Xu Y.Y., Zhao H. (2007). Improvement of xylanase production byPenicillium oxalicum ZH-30 using response surface methodology. Enzyme Microb. Technol., 40: 1381–1388.
Liu Y.T., Xuan S.X., Long C.N., Long M.N., Hu Z. (2008). Screening, identifying of cellulose-decomposing strain L-06 and its enzyme-producing conditions. Chinese J. Biotech., 24 (6): 1112–1116.
Lu M.Y., Brooks J.D., Maddox I.S. (1997). Citric acid production by solid state fermentation In a packed bed reactor usingAspergillus niger. Enzyme Microb. Technol., 21 (6): 392–397.
Ma A.Y.M., Ooraikul B. (1986). Optimization of enzymatic hydrolysis of canla meal with response surface methology. J. Food Process Pres., 10: 99–113.
Mandels M., Weber J. (1969). The production of cellulases. Adv. Chem. Se., 95: 394–414.
Nath A., Chattopadhyay P.K. (2007). Optimization of oven toasting for improving crispness and other quality attributes of ready to eat potato-soy snack using response surface methodology. J. Food Eng., 80: 1282–1292.
Ögel Z.B., Yarangümeli K., Dündar H., Ifrij I. (2001). Submerged cultivation ofScytalidium thermophilum on complex lignocellulosic biomass for endoglucanase production. Enzyme Microb. Technol., 28 (7–8): 689–695.
Panagiotou G., Kekos D., Macris B.J., Christakopoulos P. (2003). Production of cellulytic and xylanolytic enzymes byFusarium oxysporum grown on corn stover in solid state fermentation. Ind. Crops Products, 18: 37–45.
Pandey A., Soccol C.R., Mitchell D. (2000). New developments in solid state fermentation: I-Bioprocesses and products. Process Biochem., 35: 1153–1169.
Reczey K., Szengyel Z., Eklund R., Zacchi G. (1996). Cellulase production byT. Reesei. Bioresour. Technol., 57: 25–30.
Romero M.D., Aguado J., González L., Ladero M. (1999). Cellulase production byNeurospora crassa on wheat straw. Enzyme Microb. Technol., 25: 244–250.
Sin H.N., Yusof S., Hamid N.S.A., Rahman RA. (2006). Optimization of enzymatic clarification of sapodilla juice using response surface methodology. J. Food Eng., 73: 313–319.
Sukumaran R.K., Singhania R.R., Mathew G.M., Pandey A. (2009). Cellulase production using biomass feed stock and its application in ligno-cellulose saccharification for bio-ethanol production. Renew. Energ., 34 (2): 421–424.
Sun X.Y., Liu Z.Y., Zheng K., Song X., Qu Y.B. (2008). The composition of basal and induced cellulase systems inPenicillium decumbens under induction or repression conditions. Enzyme Microb. Technol., 42: 560–567.
Sun Y., Cheng J.Y. (2002). Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour. Technol., 83 (1): 1–11.
Vasconcelos A.F.D., Barbosa A.M., Dekker R.F.H., Scarminio I.S., Rezende M.I. (2000). Optimization of laccase production byBotryosphaeria sp. In the presence of veratryl alcohol by the response-surface method. Process Biochem., 35: 1131–1138.
Wen Z.Y., Liao W., Chen S.L. (2005). Production of cellulase by Trichoderma reesei from dairy manure. Bioresour. Technol., 96: 491–499.
Xia L.M., Cen P.L. (1999). Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry. Process Biochem., 34(9): 909–912.
Yang Y.H., Wang B.C., Wang Q.H., Xiang L.J., Duan C.R. (2004). Research on solid state fermentation on rice chaff with a microbial consortium. Colloid Surf., 34: 1–6.
Author information
Authors and Affiliations
Corresponding author
Additional information
An erratum to this article can be found at http://dx.doi.org/10.1007/s13213-010-0067-7
Rights and permissions
About this article
Cite this article
Long, C., Ou, Y., Guo, P. et al. Cellulase production by solid state fermentation using bagasse withPenicillium decumbens L-06. Ann. Microbiol. 59, 517–523 (2009). https://doi.org/10.1007/BF03175140
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03175140