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Purification and characterisation of a highly thermostable extracellular protease fromBacillus thermantarcticus, strain M1

Annals of Microbiology volume 58, pages 253–259 (2008)Cite this article

Abstract

A high thermostable extracellular protease was purified to homogeneity and characterised fromBacillus thermantarcticus, strain M1. The molecular mass was about 42 kDa. Almost total inhibition of protease by phenyl methyl sulphonylfluoride (PMSF), suggested that the enzyme belonged to the serine protease family. The enzyme was active and stable in a broad range of pH with an optimum at pH 7.0. The protease showed the highest activity at 70°C and was stable for 24 h at 70°C, with an increase of the enzymatic activity of about 4 times, in the presence of CaCl2. The protease retained about 50% activity after 3 h of incubation in the presence of CaCl2 with various commercial detergents. Purified protease was found to be stable, for one week, in presence of DMSO, methanol, ethanol, acetonitrile, isopropanol.

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Authors and Affiliations

  1. Istituto di Chimica Biomolecolare, CNR, Via Campi Flegrei 34, 80078, Pozzuoli (NA), Italy

    Laura Dipasquale, Valeria Calandrelli, Ida Romano, Barbara Nicolaus, Agata Gambacorta & Licia Lama

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  1. Laura Dipasquale
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  2. Valeria Calandrelli
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  3. Ida Romano
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  4. Barbara Nicolaus
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  5. Agata Gambacorta
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  6. Licia Lama
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Correspondence to Licia Lama.

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Dipasquale, L., Calandrelli, V., Romano, I. et al. Purification and characterisation of a highly thermostable extracellular protease fromBacillus thermantarcticus, strain M1. Ann. Microbiol. 58, 253–259 (2008). https://doi.org/10.1007/BF03175325

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  • DOI: https://doi.org/10.1007/BF03175325

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Annals of Microbiology

ISSN: 1869-2044

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