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  • Ecological and Environmental Microbiology
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Biological control ofFusarium wilt of pigeonpea byPantoea dispersa, a field assessment

Annals of Microbiology volume 58, pages 411–419 (2008)Cite this article

Abstract

Fusarium wilt is one of the major yield and growth-limiting factors of pigeonpea (Cajanus cajan). For an ecofriendly and sustainable management of such a disease, a novel mycolytic strainPantoea dispersa was evaluated against fungal pathogenFusarium udum that is known to be infecting the susceptible variety of pigeonpea (T-15-15), commonly prevalent in India. To study the efficacy ofP. dispersa as a biocontrol agent in comparison with chemical fungicide Bavistin and antifungal biocontrol agentTrichoderma Monitor WP was studied in both pot and field experiments.In vitro, P. dispersa inhibits the growth ofF. udum. In the pot experiment,P. dispersa the treated pigeon pea (T-15-15) seeds showed higher percentage of seed germination and decreased wilt incidence as compared to chemical fungicide; Bavistin and antifungal biocontrol agentTrichoderma Monitor WP treatments. Moreover, the root, shoot lengths and growth were also found to be higher. Due to high impact ofP. dispersa inin vitro condition on controlling theF. udum growth, further study was conducted in field in a wilt-stricken plot. These studies were repeated for three cropping seasons (2004/2007). The seed dressing byP. dispersa reduced wilt incidence (47%) during field trials, which is greater than Bavistin (41%) andTrichoderma Monitor WP (36%) treatments. HenceP. dispersa was proven superior toTrichoderma Monitor WP and Bavistin; which are used as commercialFusarium wilt control agents.

References

  • Bapat S., Shah A.K. (2000). Biological control ofFusarium wilt of pigeon pea byBacillus brevis. Can. J. Microbiol., 46: 125–132.

    Article  CAS  PubMed  Google Scholar 

  • Benitez T., Delgado-Jarana J., Rincon A.M., Rey M., Limon M.C. (1998). Biofungicides:Trichoderma as a biocontrol agent against phytopathogenic fungi. In: Pandalai S.G., Ed., Recent Research Developments in Microbiology, vol. 2. Research Signpost, Trivandrum, pp. 129–150.

    Google Scholar 

  • Bora T., Ozaktan H., Gore E., Aslan E. (2004). Biological control ofFusarium oxysporum f. sp.melonis by wettable powder formulations of the two strains ofPseudomonas putida. J. Phytopathol., 152: 471–475.

    Article  Google Scholar 

  • Deshpande M.V. (1999). Mycopesticide production by fermentation: potential and challenges. Crit. Rev. Microbiol., 25: 229–243.

    Article  CAS  PubMed  Google Scholar 

  • Dubey S.C., Suresh M., Birendra S. (2007). Evaluation ofTrichoderma species againstFusarium oxysporum f. sp.ciceris for integrated management of chickpea wilt. Biol. Control, 40: 118–127.

    Article  Google Scholar 

  • Emmert E.A.B., Handelsman J. (1999). Biocontrol of plant disease: a Gram positive perspective. FEMS Microbiol. Lett., 171: 1–9.

    Article  CAS  PubMed  Google Scholar 

  • Giesler L. J., Yuen G.Y. (1998). Evaluation ofStenotrophomonas maltophilia strain C3 for biocontrol of brown patch disease. Crop Protect., 17: 509–513.

    Article  Google Scholar 

  • Gohel V. (2006). Studies on chitinase based biocontrol of pigeonpea wilt byPantoea dispersa. PhD Thesis, M.S. University of Baroda, Vadodara, Gujarat, India.

    Google Scholar 

  • Gohel V., Chaudhari T., Vyas P., Chhatpar H.S. (2004a). Isolation and identification of marine chitinolytic bacteria and their potential in antifungal biocontrol. Indian J. Exp. Biol., 42: 715–720.

    PubMed  Google Scholar 

  • Gohel V., Megha C., Vyas P., Chhatpar H.S. (2004b). Strain improvement of chitinase producing isolatePantoea dispersa for enhancing its biocontrol potential against fungal plant pathogens. Ann. Microbiol., 54: 503–515.

    CAS  Google Scholar 

  • Gohel V., Jiwan D., Tandel S., Vyas P., Chhatpar H.S. (2005a). Management of the chitinous wastes by production of chitosan and its application. Environmental Degradation and Management, Vol. 35–42, ISBN-81-85543-0504.

  • Gohel V., Jiwan D., Vyas P., Chhatpar H.S. (2005b) Statistical optimization of chitinase production byPantoea dispersa to enhance degradation of crustacean chitin waste. J. Microbiol. Biotechnol., 15: 197–201.

    CAS  Google Scholar 

  • Gohel V., Chaudhary T., Vyas P., Chhatpar H.S. (2006a). Statistical screening of medium components for the production of chitinase by the marine isolatePantoea dispersa. Biochem. Eng. J., 28: 50–56.

    Article  CAS  Google Scholar 

  • Gohel V., Singh A., Maisuria V., Phandnis A., Chhatpar H.S. (2006b). Bioprospecting and antifungal potential of chitinolytic microoganims. African J. Biotechnol., 5: 54–72.

    Google Scholar 

  • Gohel V., Maisuria V., Chhatpar H.S. (2007). Utilization of various chitinous sources for production of mycolytic enzymes byPantoea dispersa in bench-top fermentor. Enzyme Microb. Technol., 40: 1608–1614.

    Article  CAS  Google Scholar 

  • Gwata E.T., Silim S.N., Mgonja M. (2006). Impact of a new source of resistance toFusarium wilt in pigeonpea. J. Phytopathol., 154: 62–64.

    Article  Google Scholar 

  • Hackman R.H. (1962). Studies on chitin V: The action of mineral acids on chitin. Australian J. Biol. Sci., 15: 526–537.

    CAS  Google Scholar 

  • Hillocks R.J., Minja E., Mwaga A., Nandy M.S., Subrahmanyam P. (2000). Diseases and pests of pigeonpea in eastern Africa: a review. Int. J. Pest Man., 46: 7–18.

    Article  Google Scholar 

  • Hoster F., Schmitz J.E., Daniel R. (2005). Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novelStreptomyces strain. Appl. Microbiol. Biotechnol., 66: 434–442.

    Article  CAS  PubMed  Google Scholar 

  • Hutson D., Miyamoto J. (1999). Fungicidal Activity: Chemical and Biological Approaches to Plant Protection, John Wiley and Sons, Chichester, New York, Chichester, New York, pp. 109–148.

    Google Scholar 

  • Kiprop E.K., Baudoin J.P., Mwang’ombe A.W., Kimani P.M., Mergeai G., Maquet A. (2002). Characterization of Kenyan isolates ofFusarium udum from pigeonpea [Cajanus cajan (L.) Millsp.] by cultural characteristics, aggressiveness and AFLP analysis. J. Phytopathol., 150: 517–525.

    Article  CAS  Google Scholar 

  • Kumar D., Dubey S.C. (2001). Management of collar rot of pea by the integration of biological and chemical methods. Indian Phytopathol., 57: 62–66.

    Google Scholar 

  • Larena I., Melgarejo P., De Cal A. (2003). Drying of conidia ofPenicillium oxalicum, a biological control agent againstFusarium wilt of tomato. J. Phytopathol., 151: 600–606.

    Article  Google Scholar 

  • Meena B., Radhajeyalakshmi R., Marimuthu T., Vidhyasekaran P., Velazhahan R. (2002). Biological control of groundnut late leafspot and rust by seed and foliar application of powder formulation ofPseudomonas fluorescens. Biocontrol Sci. Tech., 12: 195–204.

    Article  Google Scholar 

  • Melent’ev A.I., Helisto P., Kuz’mina L.Y., Galimzyanova N.F., Aktuganov G.E., Korpela T., (2006). Use of antagonistic bacilli for biocontrol of fungi degrading fresh wood. Appl. Biochem. Microbiol., 42: 62–66.

    Article  CAS  Google Scholar 

  • Mathre D.E., Cook R.J., Callan N.W. (1999). From discovery to use: traversing the world of commercializing biocontrol agents for plant disease control. Plant Dis., 83: 972–983.

    Article  Google Scholar 

  • Monreal J., Reese E.T. (1969). The chitinase ofSerratia marcescens. Can. J. Microbiol., 15: 689–696.

    CAS  PubMed  Google Scholar 

  • Morrissey J.P., Dow J.M., Mark G.L., O’Gara F. (2004). Are microbes at the root of a solution to world food production? EMBO Rep., 5: 922–926.

    Article  CAS  PubMed  Google Scholar 

  • Patel B., Gohel V., Raol B. (2007). Statistical optimisation of medium omponents for chitinase production byPaenibacillus sabina strain JD2. Ann. Microbiol., 57: 589–597.

    Article  CAS  Google Scholar 

  • Pleban S., Ingel F., Chet I. (1995). Control ofRhizoctonia solani andSclerotium rolfsii in the greenhouse using endophyticBacillus sp. Eur. J. Plant Pathol., 101: 665–672.

    Article  Google Scholar 

  • Poddar R.K., Singh D.V., Dubey S.C. (2004). Integrated application ofTrichoderma harzianum mutants and carbendazim to manage chickpea wilt (Fusarium oxysporum f. sp.ciceri). Indian J. Agric. Sci., 74: 346–348.

    Google Scholar 

  • Raju N.S., Niranjana S.R., Janardhana G.R., Prakash H.S., Shetty H.S., Mathur S.B. (1999). Improvement of seed quality and field emergence ofFusarium moniliforme infected sorghum seeds using biological agents. J. Sci. Food Agri., 79: 206–212.

    Article  CAS  Google Scholar 

  • Reddy M.V., Sharma S.B., Nene Y.L. (1990). Pigeonpea: diseases management. In: Nene Y.L., Hall S.D., Sheila V.K., Ed., The Pigeonpea. CAB International, Oxon, pp. 303.

    Google Scholar 

  • Tilman D., Kenneth G.C., Cassman K.G., Matson P.A., Polasky S. (2002). Agricultural sustainability and intensive production practices. Nature, 418: 671–677.

    Article  CAS  PubMed  Google Scholar 

  • Vidhyasekaran P., Muthamilan M. (1995). Development of formulations ofPseudomonas fluorescens for control of chickpea wilt. Plant Dis., 79: 782–786.

    Google Scholar 

  • Vidhyasekaran P., Sethuraman K., Rajappan K., Vasumathi K. (1997). Powder formulations ofPseudomonas fluorescens to control pigeonpea wilt. Biol. Control, 8: 166–171.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, 390 002, Vadodara, India

    Vimal B. Maisuria, Vipul Gohel & Hari S. Chhatpar

  2. Zonal Agricultural Research Station, Navsari Agriculture University, Maktampur, Bharuch, India

    Ashvinkumar N. Mehta & Rakesh R. Patel

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  1. Vimal B. Maisuria
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  2. Vipul Gohel
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  3. Ashvinkumar N. Mehta
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  4. Rakesh R. Patel
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  5. Hari S. Chhatpar
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Correspondence to Vipul Gohel.

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Maisuria, V.B., Gohel, V., Mehta, A.N. et al. Biological control ofFusarium wilt of pigeonpea byPantoea dispersa, a field assessment. Ann. Microbiol. 58, 411–419 (2008). https://doi.org/10.1007/BF03175536

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  • DOI: https://doi.org/10.1007/BF03175536

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Annals of Microbiology

ISSN: 1869-2044

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