Table 1 Primers used for amplification of the bsh gene fragments used in this study
From: Diversity of bile salt hydrolase activities in different lactobacilli toward human bile salts
Primer | Nucleotide sequence(5′–3′) | Use |
|---|---|---|
bsh-Lp1f | TGTATTTTAGTAGGTATTTCAAGCATCTC | Clone Lactobacillus plantarum bsh1 |
bsh-Lp1r | CAATGAAATGGTTACGATTACGC | Clone L. plantarum bsh1 |
bsh-Lp2f | GCTTTTTTGAGTTACTGCTTTTCTG | Clone L. plantarum bsh2 |
bsh-Lp2r | GATGAGTTTCCCCAGCTTGTT | Clone L. plantarum bsh2 |
bsh-Lp3f | ATCATTGAAAGTGCTATTCTGCC | Clone L. plantarum bsh3 |
bsh-Lp3r | CGATGACGTTACGATTAAAAACT | Clone L. plantarum bsh3 |
bsh-Lp4f | ATGAGTTCCATCCGACCATAAAT | Clone L. plantarum bsh4 |
bsh-Lp4r | AGGTCTTGTTCGGCTATTTGC | Clone L. plantarum bsh4 |
bsh-Lgf | ATTTTTACTCGCGATCGTGAACCTA | Clone Lactobacillus gasseri bsh |
bsh-Lgr | AGCTATTGCAGATGGTGACCTTGGT | Clone L. gasseri bsh |
bsh-laAf | TACAACTATTCATTTAGACGCAATATCC | Clone Lactobacillus acidophilus bshA |
bsh-laAr | CACTCTGCCAACACTCCATAACG | Clone L. acidophilus bshA |
bsh-laBf | CAAAAGCCATTTATTCCGACTGA | Clone L. acidophilus bshB |
bsh -laBr | CATAATTTATTACTTCCTTTGTTAGACAGC | Clone L. acidophilus bshB |
bsh-lsf | ATGAAATGTGTACAAGTGTTAGTGT | Clone Lactobacillus salivarius bsh |
bsh-lsr | TTTATTAACTTAACATTTGTGGA | Clone L. salivarius bsh |
bsh-lcf | GCCATTAAGCAATTCGGGTTATA | Clone Lactobacillus casei bsh |
bsh-lcr | CCAATGATTGGTCTCTCGTTCA | Clone L. casei bsh |