Table 2 Chromosomal regions of Salmonella enterica serovars Typhimurium LT2 and Typhi CT18 used to create primers for multiplex PCR (Porwollik et al. 2004)
From: Two five-plex PCRs methods for identification of common Salmonella spp. serotypes
Assay | NCBI accession no. | Primer sequence (5′ → 3′) | Amplicon size (bp) |
|---|---|---|---|
STM 1 | AE008729 | 5′-AACCGCTGCTTAATCCTGATGG-3′ | 187 |
5′-TGGCCCTGAGCCAGCTTTT-3′ | |||
STM 2 | AE008758 | 5′-TCAAAATTACCGGGCGCA-3′ | 171 |
5′-TTTTAAGACTACATACGCGCATGAA-3′ | |||
STM 3 | AE008735 | 5′-TCCAGTATGAAACAGGCAACGTGT-3′ | 137 |
5′-GCGACGCATTGTTCGATTGAT-3′ | |||
STM 4 | AE008913 | 5′-TGGCGGCAGAAGCGATG-3′ | 114 |
5′-CTTCATTCAGCAACTGACGCTGAG-3′ | |||
STM 5 | AE008913 | 5′-TGGTCACCGCGCGTGAT-3′ | 93 |
5′-CGAACGCCAGGTTCATTTGT-3′ | |||
STY 1 | AL627266 | 5′-TGGTATGGTTAAGCGGAGAATGG-3′ | 301 |
5′-GAGAGTCATAGCCCACACCAAAG-3′ | |||
STY 2 | AL627273 | 5′-GGCTGGAGCAGCCTTACAAAA–3′ | 262 |
5′-AAGAGTTGCCTGGCTGGTAAAA-3′ | |||
STY 3 | AL627273 | 5′-AATCCCCCCCCCTCAAAAA-3′ | 220 |
5′-GGTACACGTTTACTGTTTGCTGGA-3′ | |||
STM 6 | AE008879 | 5′-ATATCTCATCGTCTCCTTTTCGTGT-3′ | 181 |
5′-GAAGGTCCGGATAGGCATTCT-3′ | |||
STM 7 | AE008795 | 5′-CATAACCCGCCTCGACCTCAT-3′ | 101 |
5′-AGATGTCGTGAGAAGCGGTGG-3′ |