Fig. 3
From: Construction and expression of rabbit neutrophil peptide-1 gene in Escherichia coli
Expression and verification of the expressed fNP-1. a SDS-PAGE analysis of the expressed fNP-1 was shown; lane M shows protein marker with the molecular weight indicated, lanes 1 and 2 show the total protein profile from the E. coli BLR(DE3)/pET3b-NP1, which were cultured in the enriched LB medium without and with IPTG induction, respectively. Lane 3 shows the purified fNP1 by Ni-NTA resin. b Western blotting analysis of the expressed fNP-1. The samples were resolved on SDS-PAGE, transformed to PVDF membrane, incubated with rat anti-NP1 antisera, probed with alkaline phosphate-conjugated anti-rat IgG, and then visualized with BCIP/NBT treatment. The recognized fNP-1 is indicated by the arrow