Fig. 4
From: Construction and expression of rabbit neutrophil peptide-1 gene in Escherichia coli
Effect of IPTG concentration and IPTG inducing time on the fNP-1 expression level of cloned BLR(DE3)/pET31b-NP1. a Lanes 1–4 show the total protein profiles of the cultures treated with 0, 0.3, 0.5, and 1.0 mM of IPTG for 12 h. b Lanes 1–8 show total protein profiles of the cultures at 0, 1, 2, 3, 4, 5, 6, and 12 h after 0.5 mM IPTG induction. The expressed fNP-1 is indicated by the arrows