Table 1 Yeasts identification by PCR-RFLP analysis of the 5.8S-ITS region. Sizes in bp
From: Isolation and molecular identification of wine yeasts from a Brazilian vineyard
Species | PCR product | Restriction fragments of 5.8S-ITS region | |||
|---|---|---|---|---|---|
Cfo I | Hae III | Hinf I | Hpa I | ||
Saccharomyces cerevisiae | 850 | 360 + 310 + 135 | 310 + 240 + 180 + 140 | 370 + 360 + 120 | 720 + 130 |
Hanseniaspora uvarum | 775 | 330 + 320 + 120 | 750 | 350 + 190 + 170 | - |
Sporidiobolus pararoseus | 650 | 300 + 300 | 640 | 265 + 175 + 170 | - |
Aureobasidium pullulans | 600 | 190 + 180 + 110 | 450 + 150 | 290 + 180 + 130 | - |
Issatchenkia orientalis | 530 | 215 + 185 + 80 + 70 | 390 + 95 | 235 + 165 + 135 | - |
Issatchenkia occidentalis | 500 | 220 + 100 + 80 | 310 + 100 + 80 | 260 + 120 + 100 | - |
Issatchenkia terrícola | 450 | 120 + 110 + 100 + 90 | 290 + 130 | 240 + 110 + 100 | - |