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Fig. 3 | Annals of Microbiology

Fig. 3

From: Optimization of catalase production and purification and characterization of a novel cold-adapted Cat-2 from mesophilic bacterium Serratia marcescens SYBC-01

Fig. 3

a Effect of pH on catalase activity (•) and stability by incubating the Cat-2 in buffer (pH 3.0-10.0) for 24 h (Δ). b Effects of temperture on catalase activity (•) and temperature stability by incubating at 0, 10, 20, 30, 40, 50, 60 and 70°C for 60 min (Δ). Values given are the means of three triplicate experiments, error bars SD. c Lineweaver–Burk plot of Cat-2. The enzyme activity was measured in 50 mM phosphate buffer (pH 7.0) and at 30°C

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