Table 2 Purification of Cat-2 from Serratia marcescens SYBC-01
Step | Total activity (U) | Total protein (mg) | Sp activity (U/mg ) | Yield (%) | Purified folds |
|---|---|---|---|---|---|
Crude extract | 584,609 | 784 | 745.7 | 100 | 1 |
Ammonia sulfate precipitation | 411,776 | 385 | 1,070.0 | 70.4 | 1.4 |
Sephacryl Tm S-200 | 241,786 | 36.7 | 6,591.8 | 41.4 | 8.8 |
Mono Q | 187,411 | 4.0 | 46,619.7 | 32.1 | 62.5 |