Table 1 Purification of recombinant His-fRv3391
Steps | Protein concentration (mg/ml) | Volume (ml) | Protein (mg) | Yield (%) |
|---|---|---|---|---|
Crude supernatant | 1.3 | 50 | 65 | 100 |
Ni-NTA column | 2.1 | 9 | 18.8 | 28.9 |
Sephadex G-75 chromatography | 1.1 | 10 | 11.0 | 16.9 |
Concentration | 3.9 | 2.5 | 9.75 | 15.0 |