Fig. 1
Effect of H2SO4 concentration in the acid pretreatment on a the subsequent level of the reducing sugar liberated in pretreatment hydrolysate, and b the degree of susceptibility of the pretreated lignocellulosic fiber to cellulase degradation (hydrolysis). Lignocellulosic fibers [3% (w/v) DS] were pretreated by various concentrations of H2SO4 at 121°C, 15 lb/in2 for 30 min, then saccharified by cellulase GC 220 (62 U/g) at 40°C, pH 6 for 72 h. Solid bars Reducing sugar level, hatched bars glucose level. The data are displayed as the mean ± 1 SD, and are derived from triplicate experiments