Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli

Table 2 Box-Wilson 2⁴ factorial central composite design for the optimization of lipase fermentation by recombinant Escherichia coli

Experiment number	Glucose (g/L)(X₃)	NaCl (g/L) (X₄)	Temperature (°C) (X₇)	Induction time (h) (X₉)	Observed lipase activity (IU/mL)^a	Predicted lipase activity (IU/mL)	Cell mass (mg/mL)^a	E/x (IU/g/mL)
1	70	7	43	4	4.21 ± 0.05	7.17	2.6 ± 0.6	1.615
2	70	7	43	2	12.97 ± 1.4	14.38	2.8 ± 0.5	4.666
3	70	7	31	4	18.93 ± 2.2	18.21	3.6 ± 0.3	5.187
4	70	7	31	2	29.65 ± 3.5	30.04	3.6 ± 0.8	8.238
5	70	3	43	4	6.04 ± 0.1	8.02	2.5 ± 0.3	2.367
6	70	3	43	2	15.72 ± 1.9	16.99	2.3 ± 0.4	6.720
7	70	3	31	4	22.03 ± 3.6	20.62	3.0 ± 0.5	7.245
8	70	3	31	2	35.35 ± 3.8	34.23	3.6 ± 0.7	9.657
9	30	7	43	4	0.021 ± 0.4	1.93	0.05 ± 0.6	0.42
10	30	7	43	2	12.93 ± 1.4	14.66	2.3 ± 0.2	5.722
11	30	7	31	4	22.65 ± 3.6	21.70	3.3 ± 0.5	6.781
12	30	7	31	2	40.24 ± 4.4	39.06	3.7 ± 0.3	10.706
13	30	3	43	4	0.04 ± 0.06	-0.02	0.8 ± 0.7	0.053
14	30	3	43	2	12.96 ± 2.7	14.48	1.9 ± 0.6	6.547
15	30	3	31	4	21.93 ± 2.3	21.32	2.3 ± 0.2	9.332
16	30	3	31	2	43.07 ± 3.9	40.45	4.0 ± 0.3	10.741
17	90	5	37	3	23.38 ± 2.6	21.57	3.0 ± 0.7	7.591
18	10	5	37	3	21.84 ± 1.8	22.55	1.7 ± 0.3	8.120
19	50	9	37	3	26.92 ± 3.8	24.73	2.1 ± 0.8	9.579
20	50	1	37	3	25.87 ± 2.6	26.96	3.0 ± 0.5	8.374
21	50	5	49	3	0.05 ± 0.3	-5.74	0.4 ± 0.2	0.140
22	50	5	25	3	26.59 ± 2.6	31.27	2.6 ± 0.6	10.227
23	50	5	37	5	2.96 ± 0.4	1.98	2.0 ± 0.6	1.451
24	50	5	37	1	28.43 ± 3.6	28.31	2.8 ± 1.5	10.084
25	50	5	37	3	34.2 ± 2.4	35.33	3.4 ± 0.5	10.006
26	50	5	37	3	35.78 ± 3.7	35.33	3.4 ±0.6	10.494
27	50	5	37	3	34.95 ± 2.3	35.33	3.2 ± 0.4	10.624
28	50	5	37	3	35.25 ± 3.5	35.33	3.4 ± 0.3	10.338
29	50	5	37	3	34.96 ± 2.7	35.33	3.4 ± 0.7	10.223
30	50	5	37	3	35.5 ± 2.6	35.33	3.5 ± 0.5	10.038
31	50	5	37	3	36.01 ± 3.5	35.33	3.6 ± 0.2	9.975
32	50	5	37	3	35.89 ± 3.7	35.33	3.4 ± 0.4	10.587

ISSN: 1869-2044