Table 3 Analysis of variance for optimization of lipase production by recombinant E. coli using Box-Wilson design

Intercept

103.5437

1

103.5437

16.4015

-110.933

-4.0499

0.000832*

X₃

0.0560

1

0.0560

0.0089

0.024

0.0942

0.926090

X₃ *2

325.1179

1

325.1179

51.4991

-0.008

-7.1763

0.000002*

X₄

20.5663

1

20.5663

3.2577

4.657

1.8049

0.088831

X₄*2

166.1063

1

166.1063

26.3114

-0.593

-5.1295

0.000084*

X₇

388.8201

1

388.8201

61.5896

8.401

7.8479

0.000000*

X₇*2

939.7712

1

939.7712

148.8609

-0.157

-12.2009

0.000000*

X₉

32.5716

1

32.5716

5.1594

11.996

2.2714

0.036401*

X₉*2

752.0353

1

752.0353

119.1233

-5.046

-10.9144

0.0000008*

X₃*X₄

7.8338

1

7.8338

1.2409

-0.017

-1.1140

0.280802

X₃*X₇

76.2758

1

76.2758

12.0822

0.018

3.4759

0.002891*

X₃*X₉

2.4380

1

2.4380

0.3862

0.033

0.6214

0.542560

X₄*X₇

30.4991

1

30.4991

4.8311

0.069

2.1980

0.042092*

X₄*X₉

3.1237

1

3.1237

0.4948

0.221

0.7034

0.491317

X₇*X₉

21.4360

1

21.4360

3.3955

0.193

1.8427

0.082885

Error

107.3224

17

6.3131