Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli

Table 4 Predicted optimum values of significant variables for lipase production by the recombinant E. coli using response surface methodology (RSM)

Variable	Range studied	RSM predicted optimum values	Lipase activity (IU/mL)
Variable	Range studied	RSM predicted optimum values	Observed	Predicted by RSM
X₃	10-90	32.4	50.261 ± 4.576	48.902
X₄	1-9	5
X₇	25-49	31.7
X₉	1-5	2.1

ISSN: 1869-2044