Table 1 Purification of pectin lyase (PL) from Geobacillus stearothermophilus Ah22
Enzyme fraction | Volume (mL) | Activity (EU/mL) | Total activity | Protein (mg/mL) | Specific activity (EU/mg) | Purification fold | |
|---|---|---|---|---|---|---|---|
EU | % | ||||||
Crude extract | 100 | 0.912 | 91.2 | 100 | 0.865 | 1.039 | - |
DEAE-Cellulose | 50 | 1. 466 | 73.3 | 80.4 | 0.243 | 6.03 | 5.80 |
Sephadex G 150 | 30 | 1.822 | 54.66 | 59.9 | 0.043 | 42.37 | 40.77 |