Table 1 Purification of β-amylase from Propionicimonas sp. ENT-18 using precipitation by ice-cold acetone
Fraction (% acetone) | Protein (mg/mL) | Activity (mU/mL) | Specific activity (mU/mg protein) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Crude extract | 38.53 ± 0.61 | 63.67 ± 3.02 | 1.66 ± 0.06 | 1.0 | 100.00 ± 0.00 |
0–20 | 8.46 ± 0.61 | 4.70 ± 1.05 | 0.57 ± 0.08 | 0.3 | 7.84 ± 1.67 |
20–40 | 15.22 ± 0.03 | 9.15 ± 1.51 | 0.61 ± 0.11 | 0.4 | 14.34 ± 2.34 |
40–60 | 8.29 ± 0.17 | 27.47 ± 1.13 | 3.31 ± 0.14 | 2.0 | 43.13 ± 2.05 |
60–80 | 10.15 ± 2.34 | nda | nd | nd | nd |